凋亡素联合不同化疗药物体外抗膀胱癌效应观察  

The in vitro anti-bladder cancer effect of Apoptin accompanied with different chemotherapy drugs

作　　者：王剑松[1] 詹辉[1] 罗群强 袁顺辉[1] 李天庆[1] 王伟[1] 刘靖宇[1] 

机构地区：[1]昆明医学院第二附属医院泌尿外科云南省泌尿外科研究所,650101 [2]昆明市东川区二院外科

出　　处：《现代泌尿生殖肿瘤杂志》2010年第5期216-219,共4页Journal of Contemporary Urologic and Reproductive Oncology

基　　金：国家自然科学基金资助项目(No.30760251)

摘　　要：目的观察凋亡素联合化疗药物吉西他滨及多西紫杉醇的体外抗膀胱癌效果,评价该蛋白的临床应用前景。方法将原核表达载体pBV220/Apoptin中的Apoptin基因克隆入真核表达载体PCDNA3后,转染人膀胱尿路上皮癌细胞株EJ,利用RT-PCR观察基因表达情况,MTT法和流式细胞仪TUNEL法检测各组细胞的凋亡率,并行统计分析。结果成功构建真核表达载体PCDNA3/Apoptin并转染膀胱癌细胞株EJ,并于转染后不同时段观察到联合用药组肿瘤细胞的抑制率与凋亡率明显高于对照组(P<0.05)。结论凋亡素与吉西他滨或多西紫杉醇联合应用可发挥协同抗膀胱癌效应,凋亡素具有良好的临床应用前景。Objective To observe the anti-bladder cancer effect of Apoptin accompanied with Gemcitabine or Docetaxel in vitro,and evaluate the clinical prospect of this protein.Methods The prokaryotic expression vector of Apoptin pBV220/Apoptin,eukaryotic expression vector PCDNA3 and bladder cancer cell line EJ,were employed.Firstly,the eukaryotic expression vector PCDNA3/Apoptin was constructed,and transfected into bladder cancer cell line EJ,then the results of RT-PCR,MTT assay and the flow cytometry（TUNEL method） were observed.The data were evaluated by statistic examination.Results The differences among constructing the eukaryotic expression vector PCDNA3/Apoptin and transfecting this vector into bladder cancer cell line EJ successfully,and high level apoptosis was observed in group of Apoptin,accompanied with Gemcitabine or Docetaxel,and the single drug groups,were statistically significant.Conclusion s Apoptin accompanied with Gemcitabine or Docetaxel can get a better anti-bladder cancer result in vitro than that of single drug,which show an excellent clinical prospect of this protein.

关 键 词：膀胱肿瘤 凋亡素 吉西他滨 多西紫杉醇 治疗 

分 类 号：R737.14[医药卫生—肿瘤]