当归芍药散提取液中芍药苷和阿魏酸在大鼠体内的血浓度测定  

Simultaneous Determination of Paeoniflorin And Ferulic Acid in Extract of Dangguishaoyao powder in Rats

作　　者：鲁建武[1] 宋金春[1] 马俊玲[1] 曾俊芬[1] 

机构地区：[1]武汉大学人民医院药学部,武汉430060

出　　处：《中国药师》2010年第11期1575-1576,1580,共3页China Pharmacist

摘　　要：目的:建立HPLC法测定当归芍药散提取液中阿魏酸和芍药苷在大鼠体内血浓度的方法。方法:大鼠给药后30 min取血,采用HPLC法对大鼠血样中阿魏酸和芍药苷进行含量测定,色谱柱:Agilent ZORBAX C_(18)柱(150 mm×4.6 mm,5μm),流动相:甲醇-0.5%冰醋酸溶液(20:80),检测波长:230 nm(芍药苷)、320 nm(阿魏酸),流速:1.0 ml·min^(-1),柱温:30℃。结果:芍药苷和阿魏酸的线性范围分别为20.2～404 ng(r=0.999 8),6.2～124 ng(r=0.999 7),方法回收率、精密度均符合要求,专属性强。结论:该方法可用于大鼠体内芍药苷、阿魏酸的含量测定。Objective： To establish a method for the determination of paeoniflorin and ferulic acid in extract of Dangguishaoyao powder in rats. Method ： Plasma was detected after the rat were administered by Dangguishaoyao powder Extract 30min, HPLC was used to determine paeoniflorin and ferulic acid with the mobile phase ：methanol-0. 5% acetic acid （20： 80）. The detection wavelengths were 230 nm for paeoniflorin,320 nm for ferulic acid,the flow rate was 1.0 ml ·min-1 and column temperature was 30 ℃. Result： The two components could be detected by this method with good recoveries and preeisions. The good linearities of paeoniflorin and ferulic acid were in the range of 20. 2-404 ng（ r = 0. 999 8 ） ,6. 2-124 ng（ r =- 0. 999 7 ） respectively. Conclusion ： Paeoniflorin and ferulie acid in extract of Dangguishaoyao powder in rats can be detected by this method.

关 键 词：当归芍药散 芍药苷 阿魏酸 HPLC 

分 类 号：R285.6[医药卫生—中药学]