Isolation and Purification of Triptolide from the Leaves of Tripterygium wilfordii Hook F  

作　　者：沈剑 何潮洪 

机构地区：[1]State Key Laboratory of Chemical Engineering( Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China

出　　处：《Chinese Journal of Chemical Engineering》2010年第5期750-754,共5页中国化学工程学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China （20576113）; Zhejiang Provincial Natural Science Foundation of China （R4090358）

摘　　要：Triptolide is an important active component of Tripterygium wilfordii Hook F (TWHF) and possesses anti-inflammatory, immunosuppressive, male anti-fertility, and anticancer properties. A new method combining different techniques, including solid-liquid extraction, liquid-liquid partition, column chromatography and high-speed counter-current chromatography (HSCCC) but avoiding the use of chloroform, was developed for the isolation and purification of triptolide from the leaves of TWHF. 48 mg of triptolide at 96.5% purity was obtained from 1 kg of air-dried leaves of TWHF.Triptolide is an important active component of Tripterygium wilfordii Hook F （TWHF） and possesses anti-inflammatory, immunosuppressive, male anti-fertility, and anticancer properties. A new method combining different techniques, including solid-liquid extraction, liquid-liquid partition, column chromatography and high-speed counter-current chromatography （HSCCC） but avoiding the use of chloroform, was developed for the isolation and purification of triptolide from the leaves of TWHF. 48 mg of triptolide at 96.5% purity was obtained from 1 kg of air-dried leaves of TWHF.

关 键 词：Tripterygium wilfordii Hook F TRIPTOLIDE high-speed counter-current chromatography 

分 类 号：TQ463.4[化学工程—制药化工] TQ028.8