妊娠期糖耐量受损与Ghrelin基因多态性的关联性研究  

Association of human Ghrelin gene polymorphism with gestational impaired glucose tolerance

作　　者：宗迪[1] 孙浩[1] 钱源[2] 姚宇峰[1] 史磊[1] 史荔[1] 黄小琴[1] 马润玫[2] 褚嘉祐[1] 

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所遗传室,昆明650118 [2]昆明医学院第一附属医院妇产科,650032

出　　处：《国际遗传学杂志》2010年第5期263-267,276,共6页International Journal of Genetics

基　　金：美国纽约中华医学基金会资助项目（CMB04-805）

摘　　要：目的 研究Ghrelin基因的多态性在汉族妇女中的分布情况,并且探讨Ghrelin基因多态性与妊娠期糖耐量受损的相关性.方法 利用限制性片段长度多态性的方法检测Ghrelin基因常见的10个多态性位点在94例妊娠期糖耐量受损者及102例正常健康人中的分布情况,并构建单倍型.结果 这10个常见多态位点在妊娠期糖耐量受损组与正常对照组中的分布差异无统计学意义（两组间等位基因频率χ^2=2.790、0.224、0.072、2.887、0.004、1.073、0.653、0.671;两组间基因型频率χ^2=2.553、0.391、0.108、4.812、0.005、3.278、1.308、3.364,P＞0.05）.单倍型SNP＋408C-SNP＋2488G-SNP＋3056C,SNP＋408A-SNP＋2488G-SNP＋3056C和SNP-1500G-SNP-1062G-SNP-994C-SNP-604G在两组中的分布差异具有统计学意义（χ^2=4.336、4.308、5.327,P＜0.05）.结论 Ghrelin基因单倍型SNP＋408C-SNP＋2488G-SNP＋3056C对妊娠期糖耐量受损起到保护作用,而单倍型SNP-1500G-SNP-1062G-SNP-994C-SNP-604G和SNP＋408A-SNP＋2488G-SNP＋3056C则对其易感.Ghrelin基因的10个多态性位点与妊娠期糖耐量受损的易感性则没有存在明显的相关性.Objective To investigate whether the polymorphism of the Ghrelin gene is associated with gestational impaired glucose tolerance and its distribution in Chinese Han population. Methods We assessed common genetic variation of the Ghrelin ten single nucleotide polymorphisms（SNP）in 94 patients with gestational impaired glucose tolerance and 102 control by using the restriction fragment length polymorphism method.. In addition, haplotype assays were conducted. Results The genotype distributions of these ten common polymorphisms in gestational impaired glucose tolerance patients were not significantly different from those of normal controls in statistics（χ^2=2.790,0.224,0.072,2.887,0.004,1.073,0.653,0.671;χ^2 =2.553,0.391,0. 108,4. 812,0. 005,3. 278,1. 308,3. 364, P 〉 0. 05）, but three haplotypes（SNP-1500G - SNP-1062G -SNP-994C - SNP-604G;SNP ＋ 408C - SNP ＋ 2488G - SNP ＋ 3056C;SNP ＋ 408A - SNP ＋ 2488G - SNP ＋3056C）of the Ghrelin gene were found to be significantly associated with it in statistics（χ^2 =4.336,4.308,5.327.P 〈0. 05）. Conclusion The above mentioned ten common polymorphisms in the Ghrelin gene were not found to be significantly associated with susceptibility to gestational impaired glucose tolerance. However, one （ SNP ＋ 408C - SNP ＋ 2488 G - SNP ＋ 3056C） of the Ghrelin haplotypes showed a protective role in geslational impaired glucose tolerance, and two（ SNP-1500G - SNP-1062G - SNP-994C - SNP-604G;SNP ＋408A - SNP ＋ 2488G - SNP ＋ 3056C） showed higher susceptibility.

关 键 词：妊娠期糖耐量受损 GHRELIN基因 多态性 限制性片段长度多态性 

分 类 号：R714.256[医药卫生—妇产科学]