虎杖大黄素抗HSV_(_2)、CVB_3病毒的作用初探  

THE PRIMARY INVESTIGATION OF EFFECTS ON HSV2 AND CVB3 VIRUS OF EMODIN OF RHIZOMA POLYGONI CUSPIDATI

作　　者：王志洁[1] 

机构地区：[1]湖北医科大学病毒研究所

出　　处：《安徽中医学院学报》1999年第3期41-44,共4页Journal of Anhui Traditional Chinese Medical College

摘　　要：目的：虎杖是一种抗病毒谱广的中药，为了了解虎杖的抗病毒性能并开发利用，抽提大黄素，观察大黄素抗ＨＳＶ２、ＣＶＢ３的药效。方法：分别以ＡＣＶ作为抗ＨＳＶ２、病毒唑作为抗ＣＶＢ３的阳性对照，利用空斑减数实验以及对ＨＳＶ２ＣＰＥ的抑制，进行此项研究。结果：在ＨＥＰ２细胞系统中，ＡＣＶ对ＨＳＶ２３３３株的直接杀灭、增殖抑制、感染阻断的ＥＤ５０依次为１６．４５、１０．８５、１８．６２μｇ／ｍｌ，大黄素的相应数值依次为１．２８、１．４０、１．４４μｇ／ｍｌ。病毒唑对ＣＶＢ３直接杀灭、增殖抑制、感染阻断的ＥＤ５０分别为６．２６、７．５５、７．５０μｇ／ｍｌ，大黄素的相应数值分别为１．５４、８．３４、３．０６μｇ／ｍｌ。结论：大黄素对ＨＳＶ２３３３株的治疗指数（ＴＩ）为ＡＣＶ的４．０、２．４、３．９倍，对ＣＶＢ３的为病毒唑的４．７、１．０、２．９倍。比两个阳性对照药效好。Objective :Rhizoma Polygoni cuspidati is a Chinese medicinal herb with large antiviral range,in order to understand and exploit it's antiviral properties,we extracted emodin of it and observed the antiherpes and anticoxsackie effects of emodin. Methods: We use ACV as positive control of antiherpes ,ribavirin as positive control of anticoxsackie,through plaque reduction assays and by way of HSV2 CPE inhibition,observe the effects of antiherpes and anticoxsackie of emodin. Results: In HEp2 cell system,the median effect dose(ED50) sofdirect virucidal activity,the inhibition effectsonthe multiplicationand infection of HSV2 333ACVare 16.45g/ml,10.85g/ml ,18.62g/ml respectively ,the corresponding figuresofemodin are 1.28g/ml ,1.40g/ml ,1.44g/ml separately.The ED50s of direct virucidal activity,the inhibition effects on the multiplication and infection of CVB3 of ribavirin are 6.26g/ml,7.55g/ml,7.50g/ml respectively,the corresponding figures of emodin are 1.54g/ml,8.34g/ml,3.06g/ml separately.HZConclusion: The treatment indices(TI) of emodin to HSV2 333 strain are 4.0,2.4,3.9 times of ACV TIs,and the TIs of emodin to CVB3 are 4.7,1.0,2.9 times of ribavirintis.It should be worth exploitation.

关 键 词：虎杖大黄素 HSV-2 CVB3 抗病毒药 实验研究 

分 类 号：R285.5[医药卫生—中药学] R286.87[医药卫生—中医学]