Anti-inflammatory Mechanism of Gamma-linolenic Acid in RAW264.7 Cells  

γ-亚麻酸在RAW264.7细胞中的抗炎症作用机制(英文)

作　　者：孔秀芹[1] 殷志敏[1] 罗兰[2] 

机构地区：[1]南京师范大学生命科学学院江苏省分子医学生物技术重点实验室,江苏南京210046 [2]南京大学生命科学学院医药生物技术国家重点实验室,江苏南京210093

出　　处：《Agricultural Science & Technology》2010年第6期1-3,共3页农业科学与技术（英文版）

摘　　要：[Objective] The aim was to investigate the anti-inflammatory effect and the mechanism of gamma-linolenic acid on lipopolysaccharide-induced RAW264.7 cells.[Method] Macrophagic system RAW 264.7 cells were cultured in vitro,when cells grew to fusion state,they were pretreated with 0,12.5,25.0,50.0 μmol/L of GLA for 4 h,and then 100 ng/ml of LPS were added to induce for 12 h or 30 min.Meanwhile,the blank control and LPS control were set.And the expression of iNOS,COX-2 and the effect of GLA on IκBα,p-JNK/SAPK（Thr183/Tyr185）,p38 MAPK,p-p38 MAPK（Thr180/Tyr182）,ERK1/2,p-ERK1/2 were detected by Western blot.[Result] GLA significantly inhibited the expression of iNOS and COX-2 in RAW264.7 cells induced by LPS,and in the range of 0-50 μmol/L of GLA,the inhibition effect was concentration-dependent（P0.05）.GLA could significantly inhibited the degradation of IκBα（P0.05）,thereby inhibited the activation of NF-κB.GLA could significantly inhibited the phosphorylation of LPS-induced JNK1/2 and ERK1/2（P0.05）,while it had not significantly effect on the phosphorylation of p38（P0.05）.[Conclusion] GLA had excellent anti-inflammation effect.The inhibition of the phosphorylation of JNK1/2,ERK1/2 and the inhibition of activation of NF-κB might be the important mechanism for the educing of its biological effect.[目的]探讨γ-亚麻酸(GLA)对脂多糖(LPS)诱导的RAW264.7细胞产生炎症介质的影响及其机制。[方法]采用体外培养的巨噬细胞系RAW264.7细胞,待细胞生长至融合状态后加入不同浓度(0、12.5、25、50μmol/L)的GLA预孵4h,利用100ng/ml的脂多糖(LPS)刺激12h或0.5h,同时设空白对照和LPS对照,利用蛋白印迹法检测诱生型一氧化氮合酶(iNOS)、环加氧酶-2(COX-2)蛋白的表达以及对IκBα、p-JNK/SAPK(Thr183/Tyr185)、p38MAPK、p-p38MAPK(Thr180/Tyr182)、ERK1/2、p-ERK1/2的影响。[结果]GLA可以显著抑制LPS诱导的RAW264.7细胞中iNOS和COX-2的蛋白表达(P<0.05),且在0～50μmol/LGLA浓度范围内存在剂量依赖关系。GLA可以显著抑制IκBα的降解(P<0.05),从而抑制NF-κB的激活。GLA可以显著抑制LPS诱导的JNK1/2以及ERK1/2的磷酸化(P<0.05),对p38的磷酸化没有显著的影响。[结论]GLA具有很好的消炎功效。抑制JNK1/2以及ERK1/2的磷酸化、抑制NF-κB的激活可能是其发挥生物学效应的重要机制。

关 键 词：Gamma-linolenic acid ENDOTOXIN INFLAMMATION 

分 类 号：S816.7[农业科学—饲料科学]