线粒体DNA突变与氨基糖甙类抗生素致聋的关系  

The relation between mitochondrial DNA mutation and aminoglycoside antibioticsinduced deafness

作　　者：张丽珊[1,2] 陆明华[1,2] 黄鹰[1,2] 周哓雷 邱定红 王为未[1,2] 

机构地区：[1]南京铁道医学院生物学教研室 [2]上海肿瘤研究所

出　　处：《中华医学遗传学杂志》1999年第3期138-140,共3页Chinese Journal of Medical Genetics

基　　金：江苏省卫生厅资助

摘　　要：目的探讨线粒体ＤＮＡ突变与氨基糖甙类抗生素致聋的关系。方法应用ＰＣＲ－ＢｓｍＡＩ酶切及ＰＣＲ产物直接银染测序，对４８例氨基糖甙类抗生素致聋散发患者外周血标本的线粒体ＤＮＡ进行分析。结果发现６例患者线粒体ＤＮＡ１２ＳｒＲＮＡ基因核苷酸第１５５５位点发生了Ａ→Ｇ突变。结论氨基糖甙类抗生素致聋有明显的个体差异，带有１５５５Ｇ的个体对氨基糖甙类抗生素的耳毒作用有高度易感性。Objective To investigate the relation between mitochondrial DNA mutation and aminoglycoside antibioticsinduced deafness. Methods Mitochondrial DNA (mtDNA) of peripheral blood from 48 sporadic cases was analysed using PCRBsmAI digestion and PCRdirect DNA silver sequencing. Results Six of 48 sporadic patients had A to G mutation in 12S rRNA gene of mtDNA at nucleotide 1555.THZConclusion The results suggest that mtDNA 1555G mutation is related to the occurring of aminoglycoside antibioticsinduced deafness which can cause genetic susceptibility to aminoglycoside antibotics ototoxicity.

关 键 词：线粒体DNA突变 氨基糖苷类 抗生素 耳聋 耳毒性 

分 类 号：R978.12[医药卫生—药品] R764.430.2[医药卫生—药学]