灵芝漆酶高产菌株的筛选及产酶诱导  

Screening for high yield laccase producting strains in Ganoderma lucidum and its laccase induction production

作　　者：姚强[1] 高兴喜[2] 宫志远[1] 王宗秀[2] 任鹏飞[1] 

机构地区：[1]山东省农业科学院土壤肥料研究所,山东济南250100 [2]鲁东大学生命科学学院,山东烟台264025

出　　处：《中国酿造》2010年第11期29-31,共3页China Brewing

基　　金：国家科技支撑计划项目(2007BAD89B09-11);山东省自然科学基金项目(Q2008D02);山东省农业良种工程项目(2007LZ06-3);山东省"食用菌技术泰山学者岗位"项目资助

摘　　要：通过对灵芝1个野生菌株和9个栽培菌株进行平板初筛和液体发酵复筛,得到一株漆酶高产菌株。以该菌株为研究对象,分别考察了不同浓度Cu2+和真菌诱导子对其产漆酶的影响。结果表明:在一定浓度范围内,Cu2+能够促进该灵芝菌株漆酶的生产,当Cu2+浓度为1.0 mmol/L时,灵芝漆酶的活性达到最高;真菌诱导子同样能够显著促进灵芝漆酶的生产,随着真菌诱导子浓度的升高,灵芝漆酶的活性也逐渐升高,当真菌诱导子浓度达到120μg/mL时,灵芝漆酶活性达到最高。A high yield laccase producing strain was screened with PDA plate containing 0.04% guaiacol in the shaking flasks from 1 wild strain and 9 cultivated strains of Ganderma lucidum. The influences of different concentrations of Cu2＋ and fungal elicitor from a mushroom pathogen strain Acremonium stricture on laccase production by this strain were explored, The results showed that Cu2＋ in a concentration range （0-2mmol/L） could promote laccase production, and the highest activity of laccase was observed at 1.0mmol/L Cu2＋. Fungal elicitor can also obviously increase laccase activity, and the highest laccase activity was obtained when elicitor level was 120ug/ml.

关 键 词：灵芝 漆酶 诱导 CU2+ 真菌诱导子 

分 类 号：Q93-331[生物学—微生物学]