缓激肽对脑胶质瘤大鼠血肿瘤屏障紧密连接相关蛋白分布和表达的影响  

Effect of bradykinin on the expression and distribution of the tight junction associated protein zonula occluden-1 in brain glioma rat

作　　者：刘丽波[1] 薛一雪[1] 马腾[1] 

机构地区：[1]中国医科大学基础医学院神经生物学教研室,沈阳110001

出　　处：《山东医药》2010年第41期4-6,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30973079;30800451;30872656;30700861);辽宁省自然科学基金资助项目(20082102);辽宁省高校科研计划资助项目(2008850);沈阳科学技术计划资助项目(1091175-1-01;1081266-9-00)

摘　　要：目的研究缓激肽(BK)对血肿瘤屏障紧密连接相关蛋白(ZO-1)分布和表达的影响,以及蛋白激酶C(PKC)在BK开放紧密连接中的作用。方法将大鼠随机分为对照组和BK 5、10、15、30、60 m in组共6组。建立C6大鼠胶质瘤模型,颈动脉给药,应用W estern b lot法检测脑肿瘤微血管上ZO-1和PKC表达水平的变化;应用免疫组织化学法检测脑肿瘤微血管上ZO-1的分布和表达水平变化。结果与对照组相比,BK组紧密连接相关蛋白ZO-1表达水平显著降低,BK 15 m in组降低最明显(P<0.01);BK作用后PKC表达水平明显增加,BK 10 m in组增加最显著(P<0.01)。结论缓激肽可通过下调ZO-1的表达开放紧密连接,PKC可能是BK开放紧密连接的调节机制之一。Objective To investigate the effect of bradykinin（BK） on the expression and distribution of the tight junction（TJ） associated protein zonula occluden-1（ZO-1） in brain glioma rat and analyze the action of protein kinase C（PKC） in the process of BK opening the TJ.Methods 96 female rats were divided into 6 groups： control group,BK 5 min group,BK 10 min group,BK 15 min group,BK 30 min group and BK 60 min group.The C6 rat brain glioma models were built and BK was pumped into the rats＇ brain via the carotid artery.Western blot assay was used to test the protein expression of ZO-1 and PKC in tumor microvessels.The distribution and expression of ZO-1 on tumor microvessels were detected by SABC immunohistochemistry.Results In contrast to control group,BK obviously decreased the protein expression of ZO-1,and the most significant decrease appeared at BK infusing for 15 min（P〈0.01）.Meanwhile,the protein expression of PKC increased significantly after BK infusing and the maximum turned up at BK action 10 min（P〈0.01）.Conclusions BK could open the TJ through down-regulating the expression of TJ associated protein ZO-1.PKC might be one of the mechanisms of BK opeining the TJ.

关 键 词：缓激肽 紧密连接蛋白 蛋白激酶C 血肿瘤屏障 

分 类 号：R739.4[医药卫生—肿瘤]