硫酸镁对蛛网膜下腔出血后急性脑血管痉挛大鼠血中一氧化氮和内皮素-1水平的影响研究  

Effect of Magnesium Sulfate on Nitric Oxide and Endothelin-1 in Rats with Acute Cerebral Vasospasm after Subarachnoid Hemorrhage

作　　者：肖健齐[1] 许晶[1] 杨福义[2] 王奕[2] 王浩岩[2] 廉晓宇[2] 刘劲睿[2] 康铁江[1] 邱丽莎[1] 

机构地区：[1]黑龙江省齐齐哈尔市第一医院神经外科,161005 [2]佳木斯大学第一附属医院

出　　处：《中国全科医学》2010年第30期3403-3405,共3页Chinese General Practice

摘　　要：目的探讨硫酸镁(MgSO4)治疗对大鼠蛛网膜下腔出血(SAH)后急性脑血管痉挛(CVS)血中一氧化氮(NO)和内皮素-1(ET-1)水平的影响。方法将128只Wistar大鼠随机分为正常对照组、假手术组、单纯SAH组和SAH+MgSO4组。采用枕大池二次注血法制作大鼠SAH后急性CVS动物模型,分别于术前1h和术后1h、6h、24h采用显微图像分析仪测量各组基底动脉直径,用硝酸盐还原酶法测定各组血清NO水平,用放免法测定各组血浆ET-1水平。结果单纯SAH组在术后基底动脉直径缩小,SAH+MgSO4组在术后基底动脉直径大于单纯SAH组,但小于正常对照组和假手术组,差异均有统计学意义(P<0.05)。单纯SAH组术后血清NO水平较正常对照组和假手术组降低;血浆ET-1水平较正常对照组和假手术组升高,差异均有统计学意义(P<0.05)。SAH+MgSO4组术后血清NO水平较单纯SAH组升高,但低于正常对照组和假手术组;血浆ET-1水平较单纯SAH组降低,但高于正常对照组和假手术组,差异均有统计学意义(P<0.05)。结论血清NO水平和血浆ET-1水平的变化和动态平衡的破坏与SAH后急性CVS有关。MgSO4的治疗能够使血清NO、血浆ET-1水平恢复正常从而起到防治SAH后急性CVS的作用。Objective To discuss the effect of magnesium sulfate （MgSO4） on levels of nitric oxide （NO） and endothelin-1 （ET-1） in rats with cerebral vasospasm （CVS） following subarachnoid hemorrhage （SAH）.Methods One hundred twenty-eight Wistar rats were divided randomly into groups A （normal control group）,B （sham operation group）,C （simple SAH group） and D （SAH＋MgSO4 group）.CVS models following SAH were established by 2-time blood injection in cisterna magna.Basilar artery diameter were determined by microscopic image analysis system,NO level by nitrate reductase method and ET-1 level by radioimmunoassay 1 h before operation and at hours 1,6,24 after operation.Results After operation,basilar artery diameter shrinked in group C;the basilar artery diameter was longer in group D than in group C,but shorter than in groups A and B,the difference was significant （P0.05）.NO level was lower in group C than in groups A and B,ET-1 higher,the difference was significant （P0.05）.NO level was higher in group D than in group C,but lower than in groups A,B,and ET-1 level lower than in group C,but higher than in groups A,B （P0.05）.Conclusion The changes and dynamic equilibrium of serum NO and plasma ET-1 are related to acute CVS.MgSO4 can make levels of NO and ET-1 return to normal,thus to prevent post-SAH CVS.

关 键 词：蛛网膜下腔出血 血管痉挛 颅内 硫酸镁 一氧化氮 大鼠 

分 类 号：R743.35[医药卫生—神经病学与精神病学]