紫杉醇对喉鳞状细胞癌Hep-2细胞株的作用  

Study on the effects of taxol on Hep2 cell lines in larynx carcinoma

作　　者：倪关森[1,2] 董烨[1,2] 王家东 

机构地区：[1]上海市第四人民医院耳鼻咽喉科 [2]上海第二医科大学附属仁济医院耳鼻咽喉科

出　　处：《临床耳鼻咽喉科杂志》1999年第6期265-267,共3页Journal of Clinical Otorhinolaryngology

摘　　要：目的：了解紫杉醇对喉鳞状细胞癌细胞株Ｈｅｐ－２生长抑制作用，为治疗喉癌提供实验依据。方法：应用肿瘤细胞培养技术，随机分组和空白对照设计，观察紫杉醇对喉癌细胞生长抑制的剂量和时间效应。培养４８ｈ后作流式细胞仪分析，观察细胞周期分布和凋亡发生率。结果：紫杉醇浓度为１×１０－８组的癌细胞全部死亡，２．５×１０－９的低浓度已能明显影响细胞的生长，５×１０－９浓度组的细胞生长受到明显抑制。细胞周期分析显示：在中等或低浓度紫杉醇作用下，Ｈｅｐ－２细胞被阻止于Ｇ０／Ｇ１期，并能诱导细胞发生凋亡。结论：喉癌细胞株Ｈｅｐ－２对紫杉醇是高度的敏感，能使细胞生长阻止于Ｇ０／Ｇ１期，并诱导细胞凋亡，为紫杉醇治疗喉癌提供可靠的实验依据。Objective: To investigate the sensitivity of Hep2 cell lines in larynx squamous cell carcinoma to the taxol in vitro and to provide experimental basis for clinical therapy. Method: Techniques of cell culture and randomized blankcontrast design were used.The degree and dosedependency of taxol which induced growth inhibition in Hep2 cell lines were observed and the alteration of cell cycle was analysed by flow cytomethy. Result: All cells died at the concentration of taxol 110-8M;growth inhibition was observed clearly at the concentration of 510-9M and 2.510-9M.Flow cytometric data showed a G0/G1 arrest and apoptosis (28.96%) in two cell lines after exposure to taxol for 48 h at low and medium concentrations. Couclusion: There is a high sensitivity of Hep2 cell to taxol in vitro.The cells show G0/G1 arrest and apoptosis.This results may provide reliable guidance for treating largnx carcinoma.

关 键 词：紫杉醇 喉癌 细胞株 细胞凋亡 喉肿瘤 药物疗法 

分 类 号：R739.650.5[医药卫生—肿瘤]