c-myc反义RNA对肾癌细胞GRC-1的体外生物学影响  

Biological affection of cmyc antisense RNA on renal cell carcinoma cell line in vitro

作　　者：程伟[1,2,3] 金明[1,2,3] 邵国兴[1,2,3] 崔大祥[1,2,3] 惠宏襄[1,2,3] 石涛 

机构地区：[1]西安医科大学第二附属医院泌尿外科 [2]第四军医大学基础部生物化学与分子生物学教研室 [3]西京医院泌尿外科

出　　处：《中华泌尿外科杂志》1999年第6期325-328,共4页Chinese Journal of Urology

摘　　要：目的探讨ｃｍｙｃ反义ＲＮＡ对肾癌细胞系ＧＲＣ１的体外生物学影响。方法利用重组基因技术构建含ｃｍｙｃ反义ＲＮＡ的真核表达载体ｐｃＤＮＡ３／ｍｙｃＡＳ，再通过脂质体介导完成重组体的细胞转染。结果与对照组相比，转染反义ＲＮＡ后的细胞体外生长速度减慢，软琼脂集落形成能力降低，部分细胞恢复生长接触性抑制；流式细胞仪显示肿瘤细胞Ｓ期百分率下降，细胞阻滞于Ｇ０／Ｇ１期；细胞ＤＮＡ合成减慢，３ＨＴｄＲ掺入率降低；原位杂交检测证实，转染后细胞内源性ｃｍｙｃ基因转录水平降低；被转染细胞在超微结构上也有相应改变。结论ｃｍｙｃ反义ＲＮＡ确能在体外实验中抑制肾癌细胞生长和逆转肿瘤恶性表型。bjective To study biological affection of cmyc antisense RNA on renal cell carcinoma cell line(GRC1) in vitro.ZMethods Based on genetic recombination techniques,an eukaryotic expression vector with cmyc antisense RNA was constructed and the recombinant was then transfected into GRC1 cell line by lipofectin. ResultsBZThe growth rate and colony formation effeciency of antisense RNA transfected cells were much suppressed. The cell cycle analysis showed that the S phase fraction was significantly lower and the cell priiferation stagnated in G0/G1 phase.The rate of DNA synthesis was reduced as shown by reduced relative 3 HTdR incorporation,the mRNA transcription of cmyc being also downregulated greatly in transfected cells.In addition,under electromicroscope, there were also many obvious morphological changes occurred in transfected cells. Conclusions cmyc antisense RNA do effectively inhibit GRC1 cell growth and reverse the malignant phenotype of tumor cell in vitro.

关 键 词：肾肿瘤 C-MYC 反义RNA GRC-1 生物学 

分 类 号：R737.11[医药卫生—肿瘤]