无毛小鼠皮肤光老化模型的建立  

Establishment of skin photoaging model in BALB/c mutant mouse

作　　者：刘洪英[1] 高仙娥 杨西[1] 李善如[1] 

机构地区：[1]军事医学科学院毒物药物研究所

出　　处：《军事医学科学院院刊》1999年第2期111-113,共3页Bulletin of the Academy of Military Medical Sciences

基　　金：国家自然科学基金

摘　　要：目的：为筛选防晒药物和评价皮肤衰老药物建立无毛小鼠皮肤光老化动物实验模型。方法：采用２８０～３２０ｎｍ中波紫外线和３２０～４００ｎｍ长波紫外线长期照射无毛小鼠皮肤，造成皮肤光老化损伤。结果与结论：无毛小鼠经紫外线照射２４周后，皮肤内自由基浓度及自由基代谢产物丙二醛（ＭＤＡ）含量明显升高，皮肤中可提取的胶原比例明显降低，与未照射组小鼠相比有显著差异（Ｐ＜０．０５）。小鼠皮肤纹理密度明显减少，纹理加深，增宽。提示该突变无毛小鼠用于光老化模型不仅具有操作简便，而且易于观察等特点。Objective: To establish skin photoaging model in BALB/c mutant mouse for the screening of sun cream and the evaluation of drugs against skin senility. Methods: BALB/c mutant mouse was used as an animal model in skin photoaging experiment to observe the degree of skin damage after its longterm exposure to UV. Results and Conclusion: Compared with the control group, there was a significant increase in free radical concentration and MDA content of skin, a decrease in the density of dermatoglyph which became deeper and wider in exposed group.

关 键 词：紫外线 丙二醛 羟脯氨酸 皮肤衰老 光老化 

分 类 号：R363.122[医药卫生—病理学]