蝙蝠葛活性成分对人肺癌A549细胞株抗增殖作用的实验研究  

Experimental studies of the menispermum dauricum on anti-proliferation of human lung cancer cell line A549

作　　者：王永刚[1] 杨万山[2] 孙抒[2] 

机构地区：[1]吉林大学白求恩第一医院,长春130021 [2]延边大学基础医学院

出　　处：《山东医药》2010年第40期3-5,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30960121);吉林省中医药管理局中医药科技项目(08sy5102)

摘　　要：目的探讨蝙蝠葛活性成分对人肺癌A549细胞株抗增殖作用及其机制。方法应用MTT法测定蝙蝠葛活性成分对人肺癌A549细胞株的生长抑制作用;通过吖碇橙(AO)/溴化乙啶(EB)染色荧光显微镜观察肿瘤细胞的形态学变化;采用流式细胞仪检测A549细胞的周期分布相;应用免疫细胞化学技术SP法检测药物处理前后增殖细胞核抗原Ki-67、Bcl-2的表达。结果蝙蝠葛活性成分对人肺癌A549细胞株有明显的抑制生长的作用,且呈现出浓度依赖性;蝙蝠葛活性成分可诱导A549细胞发生细胞周期阻滞;蝙蝠葛活性成分作用后Ki-67和Bcl-2阳性表达率较对照组降低(P<0.01)。结论蝙蝠葛活性成分在体外对人肺癌A549细胞株有显著的抑制增殖作用,可能与下调Ki-67、Bcl-2蛋白表达,细胞周期发生G0/G1期阻滞有关。Objective To study the effects of the menispermum dauricum on anti-proliferation and its possible mechanism of human lung cancer cell line A549.Methods MTT assay was used to determine the effects of menispermum dauricum on A549 growth inhibitory.Morphological changes of the apoptosis of A549 were observed by using fluorescent microscope.Flow cytometry was used to detected cell cycle distribution of A549.The protein expression of Ki-67 and Bcl-2 was determined by immunocytochemical SP staining technique.Results The menispermum dauricum could inhibit the hyperplasis of A549 cell line in a dose and time dependent manner.The menispermum dauricum could arrest cell cycle in G0/G1 phase.Expression of Ki-67 and Bcl-2 in the menispermum dauricum group were lower than those in control group（P0.01）.Conclusion The proliferation of human lung cancer cell line A549 was inhibited significantly by the menispermum dauricum in vitro,which might be related with arresting cell cycle in G0/G1 phase induced by down-regulation of Ki-67 and Bcl-2.

关 键 词：蝙蝠葛活性成分 肺肿瘤 细胞增殖 KI-67抗原 基因 BCL-2 

分 类 号：R734.2[医药卫生—肿瘤]