人脐带间充质干细胞对大鼠脑出血后脑内IL-1β的影响  

Effect of human umbilical cord derived mesenchymal stem cells on IL-1β follwing intracerebral hemorrhage in rat brain

作　　者：谢姜[1] 仲健[2] 龚伟[2] 王继明[2] 任贺[2] 韩忠朝[1,2] 

机构地区：[1]中国医学科学院中国协和医科大学血液学研究所,天津300020 [2]细胞产品国家工程研究中心

出　　处：《山东医药》2010年第40期31-33,共3页Shandong Medical Journal

基　　金：国家高技术发展计划(863计划)干细胞与组织工程重点项目(2006AA02A110);天津市科技创新专项资金项目(08FDZDSH02900);天津重大支撑项目(08ZCKFGX09400)

摘　　要：目的观察人脐带间充质干细胞移植对脑出血后大鼠脑内白细胞介素-1β(IL-1β)的影响。方法胶原酶VII制作大鼠脑出血模型,实验组采用人脐带间充质干细胞移植治疗。通过ELISA和免疫组织化学方法检测脑出血损伤后3 d和7 d大鼠脑内IL-1β含量的变化。结果与对照组比较,实验组在脑出血损伤后3 d和7 d脑内IL-1β含量均减少。结论人脐带间充质干细胞能够减少大鼠脑出血后脑内IL-1β的含量,进而减轻炎症反应。Objective To investigat the effect of human umbilical cord derived mesenchymal stem cells（UC-MSCs） on interneukin-1β（IL-1β） in intracerebral hemorrhage rat.Methods An intracerebral hemorrhage（ICH） rat model was established by injection of bacterial collagenase VII into the right striatum and UC-MSCs were intracerebrally transplanted into rat peri-ICH area stereotaxically enzyme-linked immunosorbent assay（ELISA） and immunohistochemistry were employed to detect the IL-1β in rat brain tissues at day 3 and 7 post-ICH.Results The content of IL-1β was reduced in peri-ICH area in UC-MSCs group as compared with PBS control group at day 3 and 7 post-ICH.Conclusions UC-MSCs could downregulate the level of IL-1β in intracerebral hemorrhage rat brains and then reduce inflammation.

关 键 词：脑出血 白细胞介素1 间充质干细胞 

分 类 号：R743.34[医药卫生—神经病学与精神病学] R318.06[医药卫生—临床医学]