马铃薯环腐病菌Real-time Taqman-PCR检测体系的建立  

Establishment of Fluorescent Quantitation PCR Detection System(Taqman) for Clavibacer michiganensis subsp.sepedonicus

作　　者：魏琪[1,2,3] 胡林双[3] 董学志[3] 闵凡祥[3] 王晓丹[3] 李凤兰[1,2,4] 郭梅[3] 芦娜[3] 李学湛[3] 

机构地区：[1]黑龙江省农业科学院博士后工作站,黑龙江哈尔滨150086 [2]东北林业大学博士后流动站,黑龙江哈尔滨150086 [3]黑龙江省农业科学院植物脱毒苗木研究所,黑龙江哈尔滨150086 [4]东北农业大学生命科学学院,黑龙江哈尔滨150030

出　　处：《中国马铃薯》2010年第5期301-305,共5页Chinese Potato Journal

基　　金：马铃薯产业创新体系(NYCYTX-15);黑龙江省科技厅重大攻关项目(GA08B102);农业部948项目(2008-Z23);黑龙江省博士后启动基金(LBH-Z07025)

摘　　要：本研究根据环腐病菌16S rRNA保守序列设计特异性引物及探针,建立了马铃薯环腐病菌实时定量荧光PCR检测体系。研究表明,该体系可以准确、稳定、定量的检测出样品中最低浓度为1fg·μL-1(即3.21×103copies·μL-)1的目的基因,检测极限可以达到几个拷贝。该检测体系可对马铃薯环腐病菌进行微量检测,对于保证马铃薯各级种薯、商品薯及其相关产品的质量,提高市场竞争力具有重要意义。According to 16 S rRNA conserved sequence of Clavibacer michiganensis subsp.sepedonicus,specific primers were designed,and fluorescent quantitation PCR detection system（Taqman） of potato ring rot was established.In the case of definite quantitative determination,the testing capability was 1 fg·μL-1（3.21 ×103 copies·μL-1） for real-time quantitative PCR（Taqman）.It could detect several copies of genes by fluorescent quantitation PCR detection system（Taqman）.The detection system can be established for trace detection of potato ring rot,ensuring the quality of seed potatoes,ware potatoes,and related potato products,thereby improving the market competitiveness.

关 键 词：马铃薯 环腐病菌 REAL-TIME Taqman-PCR 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]