外燥对小鼠肺组织水通道蛋白1表达的影响  

Effects of External Dryness on the Expression of Aquaporin 1 in Lung of Mice

作　　者：丁建中[1] 张六通[2] 李军川[1] 胡利群[1] 张燕翔[1] 

机构地区：[1]长江大学医学院,湖北荆州434023 [2]湖北中医学院,湖北武汉430061

出　　处：《时珍国医国药》2010年第10期2669-2670,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30672572);湖北省自然科学基金资助项目(No.2009CDB289)

摘　　要：目的观察外燥对小鼠肺组织水通道蛋白1(AQP1)表达的影响。方法 60只SPF级昆明种小鼠随机分为常温常湿组、常温燥组、温燥组、凉燥组,每组15只。图像分析免疫组化法之各组第6天、第12天,第18天小鼠肺组织AQP1阳性表达率。结果各组AQP1阳性表达部位多在肺泡上皮处;温燥组第12天、第18天AQP1蛋白表达阳性率持续下降,凉燥组也呈同样趋势,尤以第12天为甚(P<0.01),伴肺部炎性病变并随时间延长而加重。结论外燥致肺部炎性病变之时,伴肺泡液细胞内外转运相关的AQP1蛋白表达下降,提示与外燥伤肺、耗津病机相关。Objective To observe the effects of External dryness Aquaporin 1（ AQP1） in lung tissue of mice . Methods 60 Kunming mice（ SPF） were divided into group A（ normal） ,group B（ normal temperature and external dryness） ,group C（ warm-Dry） , group D （ Cool-Dry） ,15 mice in each group. The expression of AQP1 was detected on day 6,12 and 18. Results Compared with group A,the positive expression of AQP1 was located in the membrane edges of alveoli,and the positive rates of AQP1 in group C and D were remarkably lowered on day 12 and 18（ P 0. 01） as the evident inflammations of lung. Conclusion The dearease of AQP1 expression is related with the damage of alveoli in external dryness.

关 键 词：外燥 小鼠 肺 水通道蛋白1 

分 类 号：R228.5[医药卫生—中医基础理论]