缺血性脑损伤中大鼠海马CA1区热休克相关因子变化的研究  

Study on change of heat shock-related factors in rat hippocampal CA1 region during ischemic brain injury

作　　者：宋远见[1] 刘红芝[1] 温相如[2] 刘永民[2] 

机构地区：[1]徐州医学院基础学院 [2]徐州医学院公共教育学院,江苏221004

出　　处：《重庆医学》2010年第21期2864-2865,共2页Chongqing medicine

基　　金：国家自然科学基金资助项目(30800446);江苏省高校自然科学研究项目(09KJB310016);徐州医学院院课题(08KJ10)

摘　　要：目的探明缺血性脑损伤过程中SD大鼠海马CA1区神经元热休克蛋白72(HSP72)mRNA和HSP72蛋白的表达情况。方法将SD大鼠随机分为假手术组、缺血再灌注30 min组、缺血再灌注3 h组、缺血再灌注12 h组、缺血再灌注1 d组、缺血再灌注3 d组、缺血再灌注5 d组和缺血再灌注7 d组。采用PT-PCR和免疫印迹方法检测SD大鼠海马CA1区在缺血再灌注后不同时期的HSP72 mRNA和HSP72蛋白表达情况。结果 HSP72 mRNA和HSP72蛋白在假手术组几乎没有表达,从再灌注3 h开始有少许表达,至再灌注3 d表达量最大,随后不断下降。结论缺血性脑损伤过程中HSP72 mRNA和HSP72蛋白在缺血再灌注不同时期有不同的表达量,在再灌注3 d时表达量达到最高峰。Objective To clear the expressing of HSP72 mRNA and HSP72 protein in rat hippocampal CA1 region during ischemic brain injury.Methods SD rats were divided randomly into 8 groups：sham-operated,cerebral ischemia and reperfusion 30 min,cerebral ischemia and reperfusion 3 h,cerebral ischemia and reperfusion 12 h,cerebral ischemia and reperfusion 1 d,cerebral ischemia and reperfusion 3 d,cerebral ischemia and reperfusion 5 d,cerebral ischemia and reperfusion 7 d.The expressing of HSP72 mRNA and HSP72 protein in hippocampus of rats was detected by PT-PCR and immunoblot assay at different time of reperfusion.Results There was no any expressing of HSP72 mRNA and HSP72 protein in sham group,some starting from cerebral ischemia and reperfusion 3 h group,peaking in cerebral ischemia and reperfusion 3 d group,and declining in cerebral ischemia and reperfusion 5 d and 7 d groups.Conclusion There is maximal expressing of HSP72 mRNA and HSP72 protein in cerebral ischemia and reperfusion 3 d group although different groups express diversely.

关 键 词：缺血性脑损伤 HSP72 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]