脑钠肽对缺血再灌注心肌细胞凋亡的影响  

The effects of BNP on myocyte apoptosis and the expression of apoptosis-associated genes in rat hearts with acute myocardial ischemia and reperfusion

作　　者：阮长武[1] 陈锐[1] 王占成[1] 蒋云[1] 沈艳[1] 袁柳[1] 李瑾[1] 

机构地区：[1]上海市第八人民医院心内科,上海200235

出　　处：《中国民康医学》2010年第21期2701-2703,共3页Medical Journal of Chinese People’s Health

摘　　要：目的:探索脑钠肽(BNP)对大鼠急性心肌缺血再灌注时心肌细胞凋亡及凋亡相关基因表达的影响。方法:采用结扎大鼠左冠状动脉法制备心肌缺血再灌注(IR)模型。将大鼠随机分为三组,(1)假手术组(Sham组)行假手术,滴注生理盐水,观察时间同实验组;(2)缺血再灌注组(IR组):缺血45分钟,再灌注1小时,滴注生理盐水;(3)脑钠肽治疗组(BNP组):缺血45分钟,再灌注1小时,滴注BNP注射液。分别以地高辛随机引物法及免疫组化法检测心肌凋亡细胞、心肌细胞bax、bcl-2基因的蛋白表达情况。结果:缺血再灌注心肌细胞凋亡数量显著高于假手术组(P<0.01),脑钠肽治疗组心肌细胞凋亡(P<0.05)明显受到抑制。IR组和BNP组bax和bcl-2基因蛋白表达均明显增加(P均<0.01),BNP明显抑制bax基因表达(P<0.05),但对bcl-2基因表达无明显影响。结论:急性心肌缺血再灌注时心肌细胞调亡加剧,bax、bcl-2参与了缺血再灌注时心肌细胞调亡调控,BNP可抑制调亡加速基因bax的表达,因而在缺血再灌注损伤心肌细胞凋亡的保护方面有一定价值。Objective：To investigate the effects of Brain natriuretic peptide（BNP） on myocyte apoptosis and the expression of apoptosis-associated genes in rat hearts with acute myocardial ischemia and reperfusion（IR）.Methods：Models were BNPde by a ligation or release of left coronary artery in rats.The rats were randomly divided into 3 groups.（1） Sham-operated group： sham surgery,infusion of normal saline,observe the same amount of time;（2） Ischemia-reperfusion group（IR group）： ischemic 45 minutes,reperfusion 1 hour,infusion of normal saline;（3）Brain natriuretic peptide in the treatment group（BNP group）： 45 minutes ischemia,reperfusion 1 hour,infusion of BNP.Myocyte apoptosis and the protein expression of bax and bcl-2 genes were detected by digoxin random labeling and immunohistochemistry.Results： The apoptotic index and the positive expression index of bax and bcl-2 genes were significantly increased in IR group（P〈0.01）.BNP significantly inhibit the myocyte apoptosis and the expression of bax gene but no effect on the bcl-2 gene expression.Conclusions： The myocyte apoptosis and the expression of bax and bcl-2 are significantly increased in rat hearts with IR.BNP is valuable in the treatment of IR because of its inhibition to bax gene expression.

关 键 词：脑钠肽 心肌细胞凋亡 缺血再灌注 

分 类 号：R-332[医药卫生] R542.22