骨桥蛋白在肝癌细胞耐受失巢凋亡中的作用  

The Significance of Osteopontin in Anoikis Resistance of Hepatocellular Carcinoma

作　　者：张岚[1] 陈漪[1] 李海玉[1] 谢晓莺[1] 沈月芳[1] 任正刚[1] 

机构地区：[1]复旦大学附属中山医院肝癌研究所,上海200032

出　　处：《中国临床医学》2010年第5期686-689,共4页Chinese Journal of Clinical Medicine

摘　　要：目的:探讨骨桥蛋白(osteopontin,OPN)在肝癌高转移细胞系MHCC-97H耐受失巢凋亡中的作用,以进一步明确OPN促进肝癌转移的机制。方法:MHCC-97H在悬浮培养条件下,观察其对失巢凋亡的敏感性。经体外化学合成骨桥蛋白序列特异性的RNAi分子(RNAi1、RNAi2、RNAi3),用脂质体(lipofectamine)转染MHCC-97H肝癌细胞系,用Western blot法检测其抑制OPN的表达的作用以及诱导失巢凋亡的作用。用流式细胞仪检测失巢凋亡情况(Annexin V/PI双染色法)。结果:在悬浮培养条件下,MHCC-97H耐受失巢凋亡。特异性的OPN RNAi转染MHCC-97H细胞后,OPN蛋白表达被显著抑制。与对照组相比,特异性RNAi转染组细胞悬浮培养72h后,凋亡率显著增加(P<0.05)。结论:OPN介导耐受失巢凋亡是高转移潜能肝癌细胞发生转移的机制之一。Objective：To clarify mechanisms of osteopontin（OPN） promoting hepatocellular carcinoma（HCC） metastasis.Methods：A high metastasis potential HCC cell line cells were cultured in suspension condition in the Poly-Hema coated dishes and the sensitivity to anoikis was observed after three specific RNAis（RNAi1,RNAi2,RNAi3） of OPN were transfected via Lipofectamine.The anoikis was measured with Flow cytometry（FCM）labeled with Annexin V/PI.Results： MHCC-97H cells were resistant to anoikis when cultured in suspension and the sensitivity to anoikis was significantly restored after OPN was inhibited with RNAi2 and RNAi3（P0.05） followed with OPN protein expression inhibition.Conclusions： The present results indicate that resistant to anoikis is one of the mechanisms for OPN promoting HCC metastasis.

关 键 词：骨桥蛋白 原发性肝癌 失巢凋亡 

分 类 号：R735.7[医药卫生—肿瘤]