γδT细胞识别人多发性骨髓瘤细胞XG-7膜热休克蛋白-70  

The recognition by human γδT cells of HSP70 peptide derived from XG 7 multiple myloma cell line

作　　者：江晓丰[1] 张学光[1] 李新燕[1] 吴士良[1] 

机构地区：[1]苏州医学院免疫研究室

出　　处：《中国免疫学杂志》1999年第4期160-162,共3页Chinese Journal of Immunology

基　　金：国家"八五"攻关项目

摘　　要：目的：探讨人多发性骨髓瘤细胞（ＭＭ）ＸＧ７选择性激发扩增人外周血γδＴ细胞机制。方法：运用蛋白质分离纯化手段获取ＭＭ细胞ＸＧ７膜蛋白组分，检查每一组分对γδＴ细胞的刺激效应，对其中的有效组分作Ｗｅｓｔｅｒｎｂｌｏｔｉｎｇ等鉴定分析。结果：证实了其中一种为热休克蛋白７０的膜蛋白能选择性扩增人外周血的γδＴ细胞。结论：表达于人多发性骨髓瘤细胞ＸＧ７细胞膜上的热休克蛋白７０多肽是γδＴ细胞所识别的配体之一。Objective:To elucidate the mechanism of γδT cell’s response to human multiple myloma(MM) cell line XG 7,and to develop a novel expansion system of γδT cell by using PBMCs stimulated with HSP70 peptide derived from XG 7.Methods:The protein isolation and purification techniques were introduced.Results:A protein which specifically stimulated γδT cells were extracted and purificated.Furthermore,this effective protein was characterized as the HSP70 molecule by western blotting.Based on the findings,successfully developed a novel culture system that selectively expanded γδT cells by using PBMCs stimulated with HPS70 peptides.Conclusion:The HSP70 peptide on XG 7cells may be one of the ligand for γδT cells.

关 键 词：ΓΔT细胞 热休克蛋白 多发性骨髓瘤 

分 类 号：R733.303[医药卫生—肿瘤] R392.12[医药卫生—临床医学]