鸡贫血病毒SJ1株DNA的分子克隆  

CLONING OF GENOMIC DNA OF CHICKEN ANEMIA VIRUS SJ1 ISOLATE IN CHINA

作　　者：陈奖励[1] 杨雨辉[1] 周方红[1] 辛九庆[1] 陈红岩[1] 刘滨东[1] 卢景良[1] 章金钢[2,3] 宋秀龙 袁吉[2,3] 何昭阳[2,3] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所 [2]中国人民解放军农牧大学 [3]吉林农业大学

出　　处：《生物技术》1999年第3期1-3,共3页Biotechnology

摘　　要：通过ＰＣＲ方法扩增，用ｐＵＣ１１９和ｐＢｌｕｅｓｃｒｉｐｔＳＫ质粒载体分段克隆了山东省分离的ＳＪ１株的全病毒ＤＮＡ。The genomic DNAof chicken anemia virus (CAV) SJ1 strain, which was isolated from Shandong province in China, was amplified by PCRwith the primers P 1,P 2 and P 3,P 4.Pland P 2 flank a 1500bp DNA fragment containing the VP 2, VP 3 and the part of VP 1 genes. P 3 and P 4 flank a 800bp DNA fragment containing the rest of RP 1 corresponding to the nucleotide sequence of Cux-1. Endonuclease analysis of the PCR-amplified DNA with the enzymes AccI, BglⅡ, PvuⅡ,PstⅠ, SacⅠ and HindⅢ showed that the restriction fragments of SJ1 strain is similar to that deduced from the nucleotide sequence from Cux-1 strain. In addition, the DNA fragment was cloned into the plasmid vectors of pUC119 and pBluescript SK respectively. It may be useful to molecular biological study of CAV isolated from China.

关 键 词：鸡贫血病病毒 聚合酶链反应 基因克隆 PCR 

分 类 号：S858.312.6[农业科学—临床兽医学]