抗除草剂草甘膦EPSPs基因在小麦中的转化  

Transformation of Common Wheat (Triticum aestivum L.) with Herbicide-resistant EPSPs Gene

作　　者：陈梁鸿[1] 王新望[1] 张文俊[2] 张晓东[3] 胡道芬[3] 刘广田[1] 

机构地区：[1]中国农业大学植物遗传育种系,北京100094 [2]中国科学院遗传研究所,北京100101 [3]北京市农林科学院植物细胞工程实验室,北京100081

出　　处：《Acta Genetica Sinica》1999年第3期239-243,共5页

摘　　要：通过基因枪法，用抗除草剂车甘膦的EPSPs基因转化小麦京花1号的幼穗约1000个，及幼胚约800个，经草甘膦选择后分别获得38株和4株再生植株。这些再生植株经PCR和（或）Southern杂交证明，其中有部分再生植株的基因组中稳定整合了外源EPSPs基因，并且转化体中部分是可育的。首次用实验证明，抗除草剂草甘膦的EPSPs基因作为单子叶禾谷类作物小麦基因转化的选择标记是行之有效的。The herbicide-resistant EPSPs (5-enolpyruvylshikimate-3-phosphate synthase) gene was transformed into about 1 000 young spikes and 800 young embryos of wheat variety, Jinghua 1, with gene gun. Thirty-eight and four regenerated plants were obtained respectively screened with glyphosate. All regenerated plants were analysed by PCR and / or Southern blotting. The results indicated that EPSPs gene was integrated stably into the genome of Jinghua 1, and some of the transformants showed fertile. So herbicide-resistant EPSPs gene could be used as selective marker in the transformation of monocotyledon cereal crops, such as wheat.

关 键 词：小麦 EPSPs基因 基因枪转化 

分 类 号：S512.103.5[农业科学—作物学]