多肽酰胺化酶的结构与功能  

Function and Structure of Peptide α Amidating Monooxygenase.

作　　者：刘深基[1] 陈松森[1] 

机构地区：[1]中国医学科学院中国协和医科大学基础医学研究所

出　　处：《生物化学与生物物理进展》1999年第3期195-197,共3页Progress In Biochemistry and Biophysics

摘　　要：Ｃ端酰胺化是许多神经内分泌多肽重要的翻译后加工过程，是在酰胺化酶的催化下分两步进行的，ＰＨＭ和ＰＡＬ分别催化这两步反应．ＰＨＭ由两个结构相似的结构域组成，每个结构域均为九链β折叠组成的三明治样结构，分别由三个Ｈｉｓ或两个Ｈｉｓ和一个Ｍｅｔ螯合一个Ｃｕ组成活性中心．在一个反应周期中，两个Ｃｕ分别独立地与抗坏血酸和分子氧发生单电子传递的氧化还原反应。Carboxy terminal amidation is an essential posttranslational modification for numerous neuronal and endocrine peptides.It is catalyzed by peptide α amidating monooxygenase (PAM),in a two step reaction . Peptidylglycine α hydroxylating monooxygenase(PHM) and peptidyl hydroxyglycine α amidating lyase(PAL) act in one of the two step.PHM catalytic core is composed of two nine stranded β sandwich domains similar in three dimessional structure.Each domain contains an activity center with a copper coordinated by three conserved His or two His and a Met residues.In the reaction cycle of producing peptidyl α hydroxyglycine,the two coppers are reduced independently by ascorbate,and they transfer one electron each to molecular oxygen.

关 键 词：多肽酰胺化酶 结构 机制 

分 类 号：Q555.3[生物学—生物化学]