抑癌基因nm23-H1在逆转录病毒载体的表达  

STUDY OF USING RETROVIRAL VECTOR FOR EXPRESSION OF TUMOR SUPPRESSOR GENE nm23-H1

作　　者：杨瑞仪[1] 张美英[1] 赵利淦[1] 

机构地区：[1]暨南大学生物工程研究所

出　　处：《病毒学报》1999年第2期185-187,共3页Chinese Journal of Virology

摘　　要：癌症转移是癌症病人死亡的主要原因之一。抑制肿瘤转移的基因ｎｍ２３－Ｈ１与ｎｍ２３－Ｈ２分别编码二磷酸核苷激酶（ＮＤＰＫ）的Ａ与Ｂ亚基〔１〕，ｎｍ２３－Ｈ１基因能有效地抑制肿瘤转移〔２〕。ｎｍ２３－Ｈ１的表达水平与黑色素瘤〔２，３〕、乳腺癌〔４〕、卵巢...The human tumor suppressor gene nm23-H1 was cloned into retroviral vector pLXSN and constructed an expressing vector pLXSN-nmh1. This recombinant plasmid was transfected into ψCRIP paking cell line by calcium phosphate precipitation technique. The colonies capable of secreting product of nm23-H1 gene and the recombinant retroviruses were selected by G418. The integration and expression of nm23-H1 gene were detected by PCR and Western blot hybrization respectively. This study demonstrates a crucial role of retroviruses as gene therapy and transgenosis.

关 键 词：逆转录病毒载体 NM23-H1 抑癌基因 

分 类 号：R730.59[医药卫生—肿瘤] Q78[医药卫生—临床医学]