灵芝糖萜微乳的制备及其对移植性肝癌Heps小鼠的抗肿瘤活性  

Preparation of Ganoderma lucidum polysaccharides and triterpenes microemulsion and its anticancer effect in mice with transplant Heps tumors

作　　者：陈彦[1] 陆慧[1,2] 宋师花[1] 贾晓斌[1] 

机构地区：[1]江苏省中医药研究院中药新型给药系统重点实验室国家中医药管理局名方适宜剂型重点研究室,江苏南京210028 [2]南京中医药大学药学院,江苏南京210046

出　　处：《中国中药杂志》2010年第20期2679-2683,共5页China Journal of Chinese Materia Medica

基　　金：江苏省公益项目(BM2007701);江苏省中医药领军人才项目(2009)

摘　　要：目的:研究灵芝糖萜复合微乳的制备工艺,同时考察其理化性质及其对移植性肝癌Heps小鼠的抗肿瘤作用。方法:采用伪三元相图得到空白微乳的处方,进而制备灵芝糖萜复合微乳;采用透射电镜及激光粒度分析仪考察糖萜微乳的形态、粒径分布和Zeta电位;测定微乳对实体瘤小鼠的抑瘤率,并比较各组实体瘤小鼠的体重、免疫器官(脾和胸腺)指数。结果:灵芝糖萜O/W型微乳的最佳处方为聚氧乙烯脱水山梨醇月桂酸酯(吐温20)-异丙醇-水-油酸体系,其比例为14.3∶14.3∶33.3∶2;透射电镜观察乳滴呈圆球形,大小均匀;平均粒径32.43 nm,Zeta电位-3.41 mV;糖萜微乳低高剂量组(57.25,114.5mg.kg-1.d-1)的抑瘤率分别为37.66%,52.34%。结论:灵芝糖萜微乳粒径小,稳定性好,对小鼠移植性肝癌Heps有显著的抑制作用。Objective： To research the microemulsion preparation of Ganoderma lucidum polysaccharides and triterpenes and investigate its properities.Evaluate the effects of polysaccharides and triterpenes microemulsions against transplant tumor growth.Method： The microemulsion formula was optimized by constructing the pseudo-ternary phase diagrams of blank microemulsion.The polysaccharides and triterpenes microemulsions were prepared on the blank microemulsions.The appearance,particle distribution and Zeta potential were investigated by transmission electron microscope and grainsize analyzer.The Heps mice were randomly administered with polysaccharides and triterpenes microemulsions（114.5,57.25 mg·kg^-1·d^-1） for 7 days.The effectiveness was assessed based on tumor inhibitory ratio of mice with Heps tumors.The toxicity was evaluated by measurements of the mice weight,immune organ weight.Result： The optimal microemulsion formula was composed of tween 20,dimethyl carbinol,water and 9-octadecenoic acid with the ratio of 14.3∶14.3∶33.3∶2.Polysaccharides and triterpenes microemulsions in transmission electron microscope were consisted of small spherical drop.The average particle size was 32.43 nm and the Zeta potential was-3.41 mV.The polysaccharides and triterpenes microemulsions showed an inhibition rate of 37.66%（57.25 mg·kg-1·d^-1）and 52.34%（114.5 mg·kg^-1·d^-1）respectively against Heps tumor growth.Conclusion： The acquired microemulsion with small particle size is stable.It significantly inhibits the tumor growth in Heps mice.

关 键 词：灵芝三萜 灵芝多糖 微乳 Heps小鼠 

分 类 号：R735.7[医药卫生—肿瘤] R285[医药卫生—临床医学]