RP-HPLC同时测定不同来源川芎中5种有效成分的含量  

Simultaneous determination of five effective components in Rhizoma Chuanxiong by RP-HPLC

作　　者：姚宗玲[1] 杜守颖[1] 陆洋[1] 徐冰[1] 陈晓兰[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中国中药杂志》2010年第20期2696-2699,共4页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2009ZX09502-008);高等学校博士学科点专项科研基金博导类课题(20090013110007)

摘　　要：目的：建立同时测定川芎中川芎嗪、阿魏酸、丁基苯酞、藁本内酯和丁烯基苯酞5种有效成分含量的RP-HPLC方法。方法：采用Grace Smart RP C18色谱柱,以乙腈-0.1%磷酸为流动相,梯度洗脱,DAD检测器检测,针对不同组分,选择其最大吸收波长下的峰面积,采用外标法定量。结果：川芎嗪、阿魏酸、丁基苯酞、藁本内酯和丁烯基苯酞的线性范围分别为0.008 350.668,0.020 61.648,0.012 20.976,0.050 754.06,0.015 71.256μg,平均加样回收率分别为101.98%,99.91%,96.94%,100.85%,99.04%。结论：该方法简便、快捷、重复性好,回收率高,可用于川芎中5种有效成分的同时测定,适用于川芎药材及其制剂的质量控制。Objective： To develop a RP-HPLC method for the simultaneous determination of five components including ligustrazine,ferulic acid,butylphthalide,ligustilide and butene-NBP in Rhizoma Chuanxiong,a Chinese herbal medicine.Method： The chromatographic analysis was carried out by using a grace smart RP C18 column with the mobile phase consisted of acetonitrile and 0.1% phosphoric acid（volume fraction）,which was in gradient elution.A DAD detector was used to detect the components.The peak area was chosen under their maximum absorption wavelength for different components,and quantitated by using the external standard method.Result： The calibration curves were linear within the range of 0.008 35-0.668,0.020 6-1.648,0.012 2-0.976,0.050 75-4.06,0.015 7-1.256 μg for ligustrazine,ferulic acid,butylphthalide,ligustilide and butene-NBP,respectively.The average recoveries of the five components were 101.98%,99.91%,96.94%,100.85% and 99.04%,respectively.Conclusion： This method is simple,quick,reproducible,with high recovery,and has been successfully applied to the simultaneous determination of the five components in Rhizoma Chuanxiong.This method can be used to control the quality of Rhizoma Chuanxiong and its preparations.

关 键 词：RP-HPLC 川芎 川芎嗪 阿魏酸 丁基苯酞 藁本内酯 丁烯基苯酞 

分 类 号：R284.1[医药卫生—中药学]