基于动物温度趋向行为学评价初步建立中药寒热体质病理模型  

Establishment of Chinese Medicine Cold and Hot Constitution Pathological Models Based on the Ethology of Animal Temperature Tropism

作　　者：周灿平[1,2] 赵艳玲[1] 王伽伯[1] 夏新华[2] 金城[1] 张学儒[1,2] 赵海平[1] 任永申[1] 肖小河[1] 

机构地区：[1]中国人民解放军第三0二医院全军中药研究所,北京市100039 [2]湖南中医药大学药学院

出　　处：《中医杂志》2010年第10期937-940,949,共5页Journal of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(“973”计划)资助项目(2007CB512607,2006CB504703);国家杰出青年科学基金资助项目(30625042)

摘　　要：目的建立寒热体质病理模型(饥寒疲劳模型、营养过剩模型),从动物行为学角度探讨不同寒热体质与小鼠温度趋向行为变化的相关性。方法将昆明种小鼠随机分3组,即正常组、饥寒疲劳组和营养过剩组,采用控制饮食+游泳法复制小鼠饥寒疲劳模型,以高蛋白饲料法复制小鼠营养过剩模型。采用冷热板示差法考察不同寒热体质小鼠温度趋向性,并通过检测ATP酶、琥珀酸脱氢酶活力(SDH)、总抗氧化能力(T-AOC)等指标,验证模型是否复制成功。结果饥寒疲劳模型动物表现出"虚寒证"特点,行为学则表现为在高温区(40℃)停留比例显著增加(P<0.05),即"趋热性"增强,其内在表现为ATP酶、SDH、T-AOC和超氧化物歧化酶(SOD)活力、机体耗氧量、环磷酸腺苷/环磷酸鸟苷(cAMP/cGMP)比值显著下降(P<0.05),丙二醛(MDA)活力和血浆cGMP含量显著增加(P<0.05),即机体能量代谢和抗氧化能力下降,营养过剩模型组表现则相反。结论基于动物温度趋向性的冷热板示差法可以直观且客观的表征饥寒疲劳与营养过剩模型的寒热体质差异,机体相关生化酶活性改变引起的能量代谢和抗氧化能力的变化可能是内在机制之一。Objective To establish the cold and hot constitution pathological models （hunger cold fatigue models, over-nutrition models） based on the ethology of animal temperature tropism to explore the correlation between the constitution and temperature tropism of mice. Methods The Kunming mice were randomized into 3 groups,i, e. normal group, hunger cold fatigue group, over- nutrition group. Food control puls swim method was used to make the hunger cold fatigue models, and high protein forage was adopted to make the over-nutrition models. An instrument with cold-hot plate was used to investigate the temperature tropism of mice, mean while, activities of adenosine triphosphatase （ATP） and succinate dehydrogenase （SDH）, and the total anti-oxidant capability （T- AOC） were determined to prove whether the models were made successfully. Results The hunger cold fatigue mice manifested with symptoms of ＂deficiency cold syndrome＂, and their behavioral manifestation was significantly increased remaining rate on warm pad （40℃） （P〈0. 05）, suggesting an enhancement of HOT tropism. Meanwhile, the ATP, SDH, T-AOC and activity of superoxide dismutase （SOD）, oxygen consumption, and the cyclic adenosine monophosphate （cAMP） / cyclic guanosine monophosphate （cGMP） decreased significantly （P〈0.05）, the activity of malondialdehyde （MDA） and the plasma cGMP increased significantly （P〈0. 05）, suggesting a lowering of energy metabolism and anti-oxidation ability. The manifestation of the over-nutrition mice was just opposite. Conclusion The cold-hot plate displaying method based on the animal temperature tropism shows directly and objectively the constitution difference of models with hunger cold fatigue and over-nutrition, the changes in the energy metabolism and anti-oxidation ability caused by the changes in the activities of the related enzymes maybe one of the internal mechanisms.

关 键 词：体质病理模型 温度趋向性 饥寒疲劳模型 营养过剩模型 

分 类 号：R-332[医药卫生]