Raf/MEK/ERK信号通路对CXCL16诱导的CIA淋巴细胞增殖的影响  

Effect of Raf/MEK/ERK signal pathway on CXCL16-induced proliferation of lymphocytes in CIA mice

作　　者：孙琳[1] 刘湘源[1] 赵金霞[1] 满斯亮[1] 张霞[1] 

机构地区：[1]北京大学第三医院风湿免疫科,北京100191

出　　处：《军医进修学院学报》2010年第11期1113-1115,1128,共4页Academic Journal of Pla Postgraduate Medical School

基　　金：国家自然科学基金项目(30772012)~~

摘　　要：目的探讨细胞外信号调节激酶(ERK)信号通路在CXCL16诱导的CIA小鼠淋巴细胞增殖中的调控作用。方法 CCK8法检测CXCL16对CIA小鼠淋巴细胞增殖的影响,ELISA法检测细胞上清中TNF-α及RANKL表达,WesternBlot检测p-Raf-1、p-ERK1/2蛋白的表达,同时观察MEK抑制剂对CXCL16诱导的上述变化的影响。结果 CXCL16对正常小鼠淋巴细胞增殖的刺激作用不明显,但CIA+CXCL16组淋巴细胞增殖能力明显高于CIA空白组及CIA+CXCL16+抑制剂组(P<0.05)。CIA+CXCL16组TNF-α水平明显高于其他各组(P<0.01)。RANKL水平在CIA+CXCL16组明显高于CIA空白组(P<0.01),但与CIA+CXCL16+抑制剂组相比无明显差别。p-Raf-1/β-actin及p-ERK1/2/β-actin在CIA+CXCL16组明显高于CIA空白组(P<0.05)。结论 CXCL16可能通过激活Raf/MEK/ERK信号通路,诱导CIA小鼠淋巴细胞增殖活化。Objective To study the effect of Raf/MEK/ERK signal pathway on CXCL16-induced proliferation of lymphocytes in CIA mice. Methods Effect of CXCL16 on proliferation of lymphocytes in CIA mice was detected by CCK8 assay. Expressions of TNF- ct and RANKL as well as p-Raf-1 and p-ERK1/2 protein in cell supernatant were detected by ELISA and Western blotting, respectively. Effect of MEK inhibitors on CXCL16-induced changes in expression of TNF- α and RANKL as well as p-Raf-1 and p-ERK1/2 protein in cell supernatant was observed. Results CXCL16 had no significant effect on proliferation of lymphocytes in normal mice. The proliferation level of lymphocytes was significantly higher in CIA＋CXCL16 group than in CIA blank and CIA＋CXCL16＋inhibitor groups （P〈0.05）. The TNF-α level was significantly higher in CIA＋CXCL16 group than in other groups （P〈0.01）. The RANKL level was significantly higher in CIA＋CXCL16 group than in CIA blank group（P〈0.01） with no significant difference between CIA＋CXCL16 and CIA＋CXCL16＋inhibitor groups. The expression level of p-Raf-1 and p-ERK1/2 was significantly higher in CIA＋CXCL16 group than in CIA blank group （P〈0.05）. Conclusion CXCL16 can induce proliferation of lymphocytes in CIA mice by activating the Raf/MEK/ERK signal pathway.

关 键 词：关节炎 实验性 淋巴细胞 细胞增殖 ERK CXCL16 

分 类 号：R739.8[医药卫生—肿瘤]