建立家兔颊黏膜鳞状上皮癌前病变模型初探  

Establishment of a model of precancerous squamous epithelial lesions in buccal mucosa of rabbits

作　　者：徐志彬[1] 吴明利[1] 王士杰[1] 尔丽绵[1] 高扬[1] 丛庆文[1] 

机构地区：[1]河北医科大学第四医院内镜室,河北石家庄050011

出　　处：《军医进修学院学报》2010年第11期1116-1118,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：河北省卫生厅2010年"医学科学研究重点课题计划"(20100432)

摘　　要：目的探讨建立与人类食管结构近似的鳞状上皮癌前病变动物模型方法。方法将含有致癌剂二甲基苯并蒽(Dimethyl-Benzanthracene,DMBA)的溶液及药膜涂抹或贴附于48只家兔口腔颊黏膜,给药16周后,做病理组织学检查和免疫组织化学检查,以12只家兔作为空白对照。结果家兔口腔颊黏膜应用二甲基苯并蒽16周后,Ⅱ-Ⅲ级不典型增生发生率达到64.6%(31/48),贴膜法所致Ⅱ-Ⅲ级不典型增生发生率达到70.8%(17/24),高于涂药法的58.3%(14/24)。免疫组化结果显示:随着病变的进展,P53、PCNA及Bcl-2的表达逐渐增强。结论应用致癌剂二甲基苯并蒽可初步构建家兔口腔颊黏膜鳞状上皮癌前病变模型,贴膜法是一种比较好的局部给药方法。Objective To establish an animal model of precancerous squamous epithelial lesions similar to human esophageal lesions. Methods Acetone solution and membrane containing dimethyl-benzanthracene（DMBA）, a carcinogenesis agent, were smeared or pasted on the buccal mucosa of 48 rabbits for 16 weeks. Then, histopathology and immunohistochemistry were used to detect changes in buccal mucosa of rabbits. Twelve rabbits were used as a blank control group. Results The rate of Ⅱ -Ⅲ grade atypical hyperplasia in buccal mucosa of rabbits was 64.6%（31/48） and 70.8%（17/24）, respectively, 16 weeks after treatment with DMBA and membrane containing DMBA. Immunohistochemistry showed that the expression levels of P53, proliferating cell nuclear antigen （PCNA） and Bcl-2 proteins in buccal mucosa of rabbits increased with the severity of precancerous lesions. Conclusion An animal model of precancerous squamous epithelial lesions in buccal mucosa of rabbits can be established with DMBA. Membrane pasting is a rather good method for local delivery of drugs.

关 键 词：颊黏膜 癌前病变 二甲基苯并蒽 

分 类 号：R73-3[医药卫生—肿瘤]