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作 者:王海彬[1] 曾展鹏[1] 谭彤燕 梁笃 杨冰 刘锋[1]
机构地区:[1]广州中医药大学第一附属医院骨科 [2]95100部队 [3]广州越秀区正骨医院
出 处:《广州中医药大学学报》2010年第6期645-648,共4页Journal of Guangzhou University of Traditional Chinese Medicine
基 金:国家自然科学基金资助项目(编号:30973761);广东省自然科学基金资助项目(编号:8151040701000016);广东省中医药管理局基金资助项目(编号:2009306;2009216)
摘 要:【目的】研究成骨细胞分化过程中线粒体生成的变化。【方法】采用Mitotracker Green染色、流式细胞仪检测成骨细胞MG-63分化过程中线粒体生成的变化;以细胞核基因组为内参,实时定量PCR检测细胞线粒体基因组数量和进一步检测细胞线粒体生成相关基因雌激素相关受体(ERRs)及其共激活因子过氧化物酶体增殖物激活受体γ共激活因子(PGC-1s)表达的变化。【结果】成骨细胞分化过程中线粒体生成显著减少,线粒体基因组数量显著下降,细胞线粒体生成相关基因ERRs及其共激活因子PGC-1s表达显著降低。【结论】成骨细胞MG-63分化过程中线粒体生成显著降低。Objective To explore the mitochondrial biogenesis in osteoblast differentiation. Methods Mitochondrial biogenesis in osteoblast differentiation was detected by Mitotracker Green staining and flow cytometric analysis. With nuclear genome as the internal standard, real time quantitative polymerase chain reaction (PCR) was applied to detect the number of mitochondrial genome. The expression of estrogen-related receptors (ERRs) and their coactivators peroxisome proliferator-activated receptor-gamma coactivator-1s (PGC-1s) which play key roles in mitochondrial biogenesis was detected by real time quantitative PCR. Results Both mitochondrial biogenesis and the number of mitochondrial genome decreased during osteoblast differentiation, and the results of real time quantitative PCR also showed that the expression of ERRs and PGC-1 s was down-regulated. Conclusion Mitochondrial biogenesis in osteoblast-like MG-63 differentiation is down-regulated.
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