广西黄皮、不同柑桔品种黄龙病检测及16S rDNA序列分析  

Detection of Citrus Huanglongbing and 16S rDNA Sequence Analysis inClausena lansium and Different Citrus Varieties from Guangxi

作　　者：邓崇岭[1] 娄兵海[1] 白先进[2] 宋雅琴[1] 陈国平[1] 赵小龙[2] 陈传武[1] 邓光宙[1] 

机构地区：[1]广西壮族自治区柑桔研究所,广西桂林541004 [2]广西壮族自治区农业厅,南宁530022

出　　处：《中国农学通报》2010年第20期283-286,共4页Chinese Agricultural Science Bulletin

基　　金：科技部国际科技合作项目"柑桔优势品种繁育及其高效栽培关键技术研究"(2008DFA30900);广西国际科技合作与交流项目"越南福泽柚子与补橙无病良种繁育技术研究与示范"(桂科合0895001-3-3);广西农业重点科技计划项目"柑桔黄龙病综合防治技术研究"(NK200827);公益性科研院所基本科研业务费"柑桔黄龙病病原研究及检测技术改进"(桂柑字[2009]45号)

摘　　要：运用nested-PCR技术对广西黄皮及11个柑桔品种进行柑桔黄龙病(citrus Huanglongbing,HLB)检测,结果表明:从黄皮及11个柑桔品种中均可扩增出一条特异性的400bp条带,说明广西种植的黄皮及11个柑桔品种均可感染HLB。同时对广西HLB病原菌16S rDNA进行扩增测序,同源性分析和聚类分析表明,广西HLB病原菌属于亚洲种,且在亚洲种不同株系中,与中国厦门株系及日本株系遗传距离较近。nested-PCR was used to detect citrus Huanglongbing （HLB） inClausena lansium and 11 citrus varieties. A specific 400 bp band was amplified from plant materials,respectively. The result demonstrated that Clausena lansium and 11 citrus varieties from Guangxi could be infected by HLB. Simultaneously,16S rDNA of HLB pathogen was amplified and sequenced. Homology analysis and cluster analysis indicated that HLB from Guangxi belonged to＇Candidatus Liberibacter asiaticus＇,and was more closed to Xiamen lines from China and two Japanese lines.

关 键 词：黄皮 柑桔 黄龙病 NESTED-PCR 16S RDNA 

分 类 号：Q789[生物学—分子生物学]