木薯生料发酵转化乙醇的工艺参数优化研究  

Optimization of Technological Parameters for Ethanol Production by Fermentation of Raw Cassava

作　　者：蔡柳 谭显胜[2] 袁哲明[1,2] 苏小军 张婷婷 李清明[1,3] 熊兴耀 

机构地区：[1]生物质醇类燃料湖南省工程实验室,长沙410128 [2]湖南农业大学生物安全科学技术学院,长沙410128 [3]湖南农业大学食品科技学院,长沙410128

出　　处：《中国农学通报》2010年第20期406-412,共7页Chinese Agricultural Science Bulletin

基　　金：国家科技支撑项目(2007BAD41B03);教育部新世纪优秀人才计划(NCET-06-0710)

摘　　要：研究不同试验设计方法对发酵工艺的影响,为中国燃料乙醇发展提供试验依据。以木薯(Manihot esculenta)为试材,分别用正交设计法和均匀设计法考察木薯生料发酵转化乙醇工艺参数,分析讨论2种设计的特点,得出在多水平多因素的条件下,均匀设计具有实施试验少、效果好等优势,并得到以乙醇产量为指标的生料发酵转化乙醇最佳工艺参数:料水比1:1.8,初始pH3.5,温度32℃,酵母添加量3.5×107cell/mL,(NH4)2SO40.5g,转速140r/min,糖化酶添加量200u/g,淀粉酶添加量12u/g,纤维素酶添加量25u/g,发酵周期120h,此时乙醇得率达到15.7%(正交设计最优结果为9.2%)。The aim was to study the influence of different design methods on fermentation technology,and provide a scientific basis for fuel ethanol development. Taking cassava as test piece,the uniform design and orthogonal design was used to study the technological parameters optimizated for raw cassava ethanol production,and the two design＇s methods characteristics were discussed. The results showed that uniform design had more advantages than orthogonal design under the conditions of multi-levels and multi-factors. The optimal technological parameters for the ethanol yield were obtained：ratio of solid to liquid 1：1.8,initial pH 3.5,fermentation temperature 32℃,yeast inoculation amount 3.5×107 cell/mL,（NH4）2SO4 0.5 g,rotational speed 140 r/min,dosage of glucoamylase,a-amylase and cellulose were 200 u/g,12 u/g and 25 u/g,fermentation cycle 120 h,under the optimal conditions,ethanol concentration could reach 15.7% （9.2% under orthogonal design）.

关 键 词：木薯 燃料乙醇 生料发酵 均匀设计 正交设计 

分 类 号：S216.2[农业科学—农业机械化工程]