温度对鳊鱼静止代谢和耐低氧能力的影响  

Temperature Effect on Rest Metabolic Rate and Hypoxia Tolerance in Chinese Bream Parabramis pekinensis

作　　者：陈波见[1] 曹振东[1] 付世建[1] 王宇翔[1,2] 

机构地区：[1]重庆师范大学进化生理与行为学实验室重庆市动物生物学重点实验室,重庆400047 [2]女王大学生物系

出　　处：《动物学杂志》2010年第5期1-8,共8页Chinese Journal of Zoology

基　　金：国家自然科学基金项目(No.30700087);重庆市高校优秀人才支持计划项目;重庆市自然科学基金项目(No.2010BB1089)

摘　　要：以鳊鱼(Parabramis pekinensis)幼鱼[体重(8.13±0.15)g,n=31]为研究对象,以密闭式代谢测定法分别在15、20、25、30℃条件下测定其静止代谢率(Rest Metabolic Rate,RMR),并以双线法推算临界氧浓度Pcrit等相关参数。结果显示,鳊鱼幼鱼的静止代谢水平呈随温度上升而升高的变化趋势[(132.22±4.97)mg O2/(h.kg)(15℃),(182.67±12.49)mg O2/(h.kg)(20℃),(218.44±9.20)mgO2/(h.kg)(25℃),(298.32±9.96)mg O2/(h.kg)(30℃)],除20～25℃间,其他各温度组差异显著(P<0.05);Q10值均低于2(15～20℃1.91,20～25℃1.43,25～30℃1.87);本研究还发现,随着温度的升高,鳊鱼幼鱼的Pcrit逐渐下降,而临界氧饱和度Scrit则相对保守[15、20、25、30℃的Pcrit分别为(1.35±0.09)、(1.16±0.19)、(0.97±0.12)和(0.86±0.09)mg O2/L,Scrit分别为13.87%±0.74%、13.32%±1.72%、12.15%±1.35%和11.34%±0.65%。这表明,采用不同指标考察鱼类耐低氧能力可能会得到相异的结论。这种结论的不一致提示,在鱼类应对环境溶氧降低的适应中,相对溶氧水平而不是绝对溶氧水平影响并决定着鱼类的适应性进化,因此采用相对饱和度作为指标研究鱼类耐低氧能力可能更具理论价值。Rest metabolic rate（RMR） was measured using sealed respirometer and critical oxygen concentration（Pcrit） was calculated using two-segmented straight lines method on Chinese Bream Parabramis pekinensis weighed 8.13 ± 0.15 g（n = 31） at 15,20,25 and 30℃.The RMR was 132.22 ± 4.97,182.67 ± 12.49,218.44 ± 9.20 and 298.32 ± 9.96 mg O2 /（h.kg） respectively at 15,20,25 and 30℃ with an ascending trend with temperature.There was a significant difference between groups（P 0.05） except for the RMR at 20 and 25℃.The Q10 value were less than 2 [1.91（15-20℃）,1.43（20-25℃）,1.87（25-30℃）]in all thegroups.The Pcrit was 1.35 ± 0.09,1.16 ± 0.19,0.97 ± 0.12 and 0.86 ± 0.09 mg O2 /L,Scrit was 13.87% ± 0.74%,13.32% ± 1.72%,12.15% ± 1.35% and 11.34% ± 0.65% at 15,20,25 and 30℃,respectively.The results revealed that Pcrit decreased as temperature increasing while Scrit was relative stable with temperature change.Our study suggests that the conclusion of study on hypoxia tolerance capability in fish would be varied with different indices measured.It also indicated that in the process of fish physiological adaptation to hypoxia,the effect of relative oxygen saturation rather than the absolute oxygen concentration played determination role.Therefore,critical oxygen saturation index may be better practical in fish hypoxia tolerance study.

关 键 词：鳊鱼 温度 静止代谢 耐低氧能力 

分 类 号：Q4[生物学—生理学]