多西环素纳米脂质体缓释凝胶改善大鼠牙周损伤的作用评价  

Doxycycline nano-liposome slow-release gel improves rat periodontitis

作　　者：金鸿莱[1] 吴伟[1] 束蓉[2] 

机构地区：[1]上海交通大学医学院附属第九人民医院.周浦分院口腔科,上海201318 [2]上海交通大学医学院附属第九人民医院.口腔医学院牙周病科,上海200011

出　　处：《上海口腔医学》2010年第5期508-511,共4页Shanghai Journal of Stomatology

基　　金：上海市科学技术委员会基金(0352nm126);南汇区科学技术委员会基金(NKRF0806)~~

摘　　要：目的:在动物体内检验多西环素纳米脂质体缓释凝胶的生物相容性及其对牙周损伤动物模型的修复效果。方法:毒性实验-用20%样品(多西环素纳米脂质体缓释凝胶)水溶液给予SD大鼠灌胃,观察长期应用多西环素纳米脂质体凝胶剂有无全身毒性反应及其程度。造模动物实验-将牙周损伤动物模型造模成功的SD大鼠分为5组(空白组、派丽奥组、纳米组、基质组、造模组),按分组进行相应治疗,通过实时定量PCR检验各组大鼠龈沟液中MMP-8及其抑制剂TIMP-1的表达,采用SAS13.3软件包对数据进行t检验。结果:自制的多西环素纳米脂质体缓释凝胶具有良好的生物相容性;经多西环素纳米脂质体缓释凝胶治疗1个月后的牙周病变大鼠龈沟液中MMP-8含量明显降低,与其他对照组相比有显著性差异(P<0.05)。结论:牙周袋内应用多西环素纳米脂质体缓释凝胶可降低龈沟液中MMP-8的含量,减轻牙周炎症,提示该药物制剂对改善大鼠牙周炎症有一定疗效。PURPOSE：To evaluate the therapeutic effects of doxycycline nano-liposome slow-release gel on the periodontitis in an established rat model.METHODS：The biocompatibility was tested by oral perfusion sample gel for long term observation.Doxycycline nano-gel was used to treat the established rat model of periodontitis.The rats were selected randomly and divided into five groups：blank group, doxycycline nano-gel treated group, minocycline-treated group, matrix group and periodontitis model group.MMP-8 and TIMP-1 in the sulcus fluid were detected by real-time PCR.The data was analysed using SAS13.3 software package for Student＇s test.RESULTS：Doxycycline nano-gel had excellent biocompatibility from weight measure and tissue section evaluation.The results in periodontitis rats demonstrated that doxycycline nano-gel could ameliorate periodontitis condition dramatically compared with the control group, the restoration even better than minocycline-treated group.CONCLUSIONS：Doxycycline nano-liposome slow-release gel improves rat periodontitis by decreasing MMP-8 level, which provides valuable evidence to promote doxycycline nano-gel clinical application.

关 键 词：纳米脂质体 凝胶缓释剂 生物相容性 大鼠 牙周炎 动物模型 

分 类 号：R781.4[医药卫生—口腔医学]