野黄芩苷对内毒素介导人牙周膜细胞表达MMP-1和MMP-2蛋白的影响  

Effects of scutellarin on LPS-mediated expression of MMP-1 and MMP-2 in human periodontal ligament cells

作　　者：葛志华[1] 张莹[2] 杨宁[1] 郭寅 李秀英[1] 焦香香[1] 孙立新[1] 

机构地区：[1]承德医学院附属医院,河北承德067000 [2]第四军医大学口腔医学院,陕西西安710032 [3]承德市口腔医院,河北承德067000

出　　处：《牙体牙髓牙周病学杂志》2010年第10期561-564,共4页Chinese Journal of Conservative Dentistry

基　　金：河北省中医药管理局2006计划课题(2006037)

摘　　要：目的:观察不同浓度野黄芩苷对内毒素(LPS)介导人牙周膜细胞表达MMP-1和MMP-2蛋白的影响。方法:原代培养人牙周膜细胞(HPDLCs),实验分为空白对照组、LPS(阳性对照)组、不同浓度野黄芩苷加LPS组。采用蛋白印迹法定量检测人牙周膜细胞中MMP-1、MMP-2蛋白表达。结果:加入100μg/mL浓度的LPS时,能上调人牙周膜细胞MMP-2蛋白表达(P<0.05),但对MMP-1蛋白表达影响不明显。同时加入0.001、0.01、0.1、1、10μg/mL不同浓度野黄芩苷后,10μg/mL浓度的野黄芩苷能降低内毒素作用的人牙周膜细胞MMP-2蛋白表达(P<0.05),但野黄芩苷降低MMP-1蛋白的作用不明显。结论:LPS能诱导人牙周膜细胞MMP-2蛋白高表达,10μg/mL野黄芩苷对LPS诱导人牙周膜细胞表达MMP-2蛋白有抑制作用。AIM：To investigate the effects of scutellarin on the expression of lipopolysaccharide（LPS）stimulated matrix metalloproteinases（MMP）-1 and MMP-2 in human periodontal ligament cells（HPDLCs）.METHODS：HPDLCs were primarily cultured in vitro.Cultured HPDLCs were treated with LPS or LPS plus different concentrations of scutellarin.Non-treated HPDLCs served as controls.Western blot was used to detect the MMP-1 and MMP-2 expression in HPDLCs.RESULTS：MMP-2 was significantly increased when stimulated by 100 μg/mL E.Coli LPS,while MMP-1was not significantly influnced.Simultaneous addition of scutellarin decreased MMP-2 expression,and 10 μg/mL of scutellarin showed significant decreasing effect（P 〈 0.05）.The same treatment showed no obvious effect on MMP-1.CONCLUSION：Scutellarin can inhibit the LPS-stimulated MMP-2 expression in HPDLCs.

关 键 词：野黄芩苷 人牙周膜细胞 内毒素 基质金属蛋白酶 

分 类 号：R780.2[医药卫生—口腔医学]