丙型肝炎病毒及恶性疟原虫复合抗原基因表达及诱导小鼠免疫应答的研究  

Expression ofaFusedGenefortheMultiple Epitopes Antigen of Hepatitis C Virus andPlasmodium falciparumand Studies onIts Immunogenicity

作　　者：解咏梅[1] 黄建生[1] 张潜 胡幼卿[1] 沈先荣[1] 张明薇 董宁[1] 董文其 郭明秋 任大明[1] 

机构地区：[1]复旦大学遗传学研究所遗传工程国家重点实验室

出　　处：《上海免疫学杂志》1999年第3期134-137,共4页Shanghai Journal of Immunology

基　　金：国家自然科学基金

摘　　要：将合成的丙型肝炎病毒（ＨＣＶ）复合多表位抗原基因ＰＣＸ及恶性疟原虫（Ｐｆ）多价抗原基因ＡＢ（ＳＰｆ６６＋ＳＰｆ１０５）克隆到表达载体ｐＷＲ４５０ｌ中，以β半乳糖甘酶（ＧＺ）ＨＣＶＰｆ复合多肽抗原的形式在大肠杆菌中高效融合表达了目的蛋白ＧＺＣＡＢ，表达量约３０％。所表达的重组抗原可被ＨＣＶ患者血清及鼠抗Ｐｆ抗体特异识别，显示了该融合蛋白质具有ＨＣＶ和Ｐｆ的免疫原性。该蛋白免疫小鼠后，诱发针对ＧＺＣＡＢ、ＧＺＰＣＸ及ＰｆＡｇ的特异性体液免疫应答及针对ＧＺＣＡＢ、ＧＺＰＣＸ的迟发性超敏反应，ＣＤ８＋Ｔ细胞明显升高，提示该复合抗原作为ＨＣＶＰｆ双价疫苗的可行性。Two synthetic multiple epitopes antigen genes, the gene PCX of hepatitis C virus(HCV) and the gene AB of Plasmodium falciparum (Pf), were cloned to galactosidase expression vector pWR4501 to highly express a fused antigen GZCAB in the form of inclusion body in E coli ,whose products accounted for about 30% of total bacterialproteins The purified GZCAB protein reacted with the patients' sera of hepatitisC and antiPf antisera, which showed its specific antigenicity of both HCV and PfWhenusedforimmunizationofmice,the protein could induced highly specific antibodies and cellularimmunityTheresultsshowthepossibilityforbivalentvaccineofhepatitisCvirusandPlasmodiumfalciparum

关 键 词：丙型肝炎 恶性疟原虫 免疫应答 融合表达 

分 类 号：R512.630.3[医药卫生—内科学]