长期能量限制增加去乙酰化酶SIRT1表达和降低小鼠血管的衰老  

Long-term calorie restriction increases SIRT1 expression and inhibits vascular senescence in mice

作　　者：周爽[1] 陈厚早[1] 万言珍[1] 张然[1] 张庆军[1] 刘德培[1] 

机构地区：[1]中国医学科学院北京协和医学院基础医学研究所医学分子生物学国家重点实验室,北京100005

出　　处：《基础医学与临床》2010年第11期1158-1162,共5页Basic and Clinical Medicine

基　　金：国家重点基础研究发展计划项目(973)(2011CB503902);国家高技术研究发展计划项目(863)(2006AA02A406);国家重点实验室专项经费(2060204)

摘　　要：目的初步研究能量限制对血管细胞衰老的影响及作用机制。方法取2月龄和18月龄的C57BL/6小鼠,用Western blot检测血管细胞衰老相关分子的变化;建立能量限制12个月的小鼠模型,用Western blot检测相关分子的蛋白水平。结果与2月龄小鼠相比,18月龄的小鼠动脉中SIRT1表达水平显著降低。能量限制显著增加小鼠动脉中SIRT1表达水平,降低细胞衰老标志分子P53和P21表达水平,增加抗氧化酶MnSOD的表达水平。结论能量限制可以抑制血管细胞衰老,其机制可能与增加SIRT1表达、降低氧化应激有关。Objective To investigate the effects of calorie restriction on vascular senescence and potential underlying mechanisms.Methods Comparing ageing-related gene expression in aortas of 2 or 18-month-old C57BL/6 mice by Western blot.Constructing calorie restriction mouse model and analyzing gene expression by Western blot using ageing-related antibodies.Results Ageing decreased the expression of SIRT1,a type III histone deacetylase,in aortas of mice.Whereas long-term calorie restriction increased SIRT1.The ageing-related biomakers P53 and P21 expression were decreased,but MnSOD expression was upregulated in aortas of C57BL/6 mice by long-term calorie restriction.Conclusion Long-term calorie restriction inhibits vascular senescence.SIRT1 is involved in vascular senescence inhibition leading to decreased oxidative stress.

关 键 词：能量限制 SIRT1 细胞衰老 氧化应激 

分 类 号：R363[医药卫生—病理学]