土槿乙酸诱导人黑色素瘤细胞凋亡  

Apoptosis induced by pseudolaric acid B in human melanoma cells

作　　者：孟爱国[1] 刘春艳[2] 

机构地区：[1]华北煤炭医学院附属医院检验科,河北唐山063000 [2]华北煤炭医学院药学系,河北唐山063000

出　　处：《基础医学与临床》2010年第11期1188-1192,共5页Basic and Clinical Medicine

基　　金：河北省自然科学基金(C2010001758)

摘　　要：目的研究土槿乙酸诱导人黑色素瘤(SK-28)细胞凋亡。方法采用Hoechst33342/PI核荧光双染色法、DNA片段化分析检测细胞凋亡;用ELISA和RT-PCR检测P53、Fas、caspase-3、BAX和BCL-2的蛋白和mRNA的表达。结果土槿乙酸明显诱导SK-28细胞凋亡(P<0.05);作用12 h后,P53的蛋白表达水平显著增加,作用24 h后,Fas/APO-1蛋白表达和BAXmRNA表达增加,BCL-2mRNA表达降低,Caspase-3活性提高。结论土槿乙酸可通过激活P53引发BCL-2介导的线粒体途径和Fas/APO-1介导的死亡受体途径,诱导人黑色素瘤SK-28细胞凋亡。Objective To investigate the effect of Pseudolaric acid B-induced apoptosis in human melanoma cells.MethodsApoptotic cells were detected using Hoechst 33342/PI staining,and confirmed by DNA fragmentation assay.The expression of genes involved in apoptosis was analysized by RT-PCR.The level of the protein was determined by ELISA.Results PLAB clearly induced apoptosis in SK-28 cells;Treatment with PLAB for 12 h,the protein expression of P53 was obviously increased;for 24 h,caspase-3 activity was enhanced,the protein expression of Fas/APO-1 was increased,and the mRNA expression of BAX was also elevated,but the mRNA expression of BCL-2 was reduced.Conclusion PLAB induced apoptosis in human melanoma cells via a mechanism involving both BCL-2-associated mitochondrial pathway and Fas-associated death receptor signaling pathway,which are two distinct downstream pathways of P53.

关 键 词：土槿乙酸 P53 FAS/APO-1 BCL-2 CASPASE-3 

分 类 号：R739.5[医药卫生—肿瘤]