Optimization of detecting hydrogenase activity for acidogenic fermentation of activated sludge  

作　　者：郑国臣 赫俊国 李建政 阿杰 张立国 

机构地区：[1]State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology [2]School of Municipal and Environmental Engineering,Harbin Institute of Technology

出　　处：《Journal of Harbin Institute of Technology(New Series)》2010年第5期721-725,共5页哈尔滨工业大学学报（英文版）

基　　金：Sponsored by the the National High Technology Research and Development Program of China(Grant No.2006AA05Z109);Harbin Municipal Scienceand Technology Innovation Talents of Special Fund Projects(Grant No.2009RFXXS004)

摘　　要：In order to evaluate the hydrogen-producing efficiency of anaerobic activated sludge in Anaerobic Baffled Reactor(ABR)fermentation processes,the optimal conditions for hydrogen producing hydrogenase method on methyl viologen(MV)assay was used to detect the hydrogen production activity of the activated sludge.The most favorable parameters such as 0.6 mL sodium acetate buffer(pH 5.0),100 μL lysozyme,0.2 mL sodium dibromoethane(9.0 mmol/L)and 0.7 mmol/L iron added into 1 mL activated sludge(2.66～26.64 gMLVSS/L)were found.Furthermore,reaction temperature and culture time were detected as 40 ℃ and 30 min respectively.Sodium thiosulfate and sodium sulfides were taken as the reducing agent while trichloroacetic acid as terminator.Under the MV optimal conditions,micro-toxic Dimethyl sulfoxide(DMSO)get higher security and better accuracy.The sensitivity of the detection methods(DMSO as electron carrier)was increased by more than 30%.The results show that the optimal conditions can be applied to measure hydrogenase activity correlating with its specific hydrogen production rate in a hydrogen-producing anaerobic activated sludge system.In order to evaluate the hydrogen-producing efficiency of anaerobic activated sludge in Anaerobic Baffled Reactor（ABR）fermentation processes,the optimal conditions for hydrogen producing hydrogenase method on methyl viologen（MV）assay was used to detect the hydrogen production activity of the activated sludge.The most favorable parameters such as 0.6 mL sodium acetate buffer（pH 5.0）,100 μL lysozyme,0.2 mL sodium dibromoethane（9.0 mmol/L）and 0.7 mmol/L iron added into 1 mL activated sludge（2.66～26.64 gMLVSS/L）were found.Furthermore,reaction temperature and culture time were detected as 40 ℃ and 30 min respectively.Sodium thiosulfate and sodium sulfides were taken as the reducing agent while trichloroacetic acid as terminator.Under the MV optimal conditions,micro-toxic Dimethyl sulfoxide（DMSO）get higher security and better accuracy.The sensitivity of the detection methods（DMSO as electron carrier）was increased by more than 30%.The results show that the optimal conditions can be applied to measure hydrogenase activity correlating with its specific hydrogen production rate in a hydrogen-producing anaerobic activated sludge system.

关 键 词：FERMENTATION ANAEROBE activated sludge HYDROGENASE 

分 类 号：X172[环境科学与工程—环境科学]