高效液相色谱法同时测定功能食品中绿原酸、白藜芦醇、葛根素和芦荟甙  

SIMULTANEOUS DETERMINATION OF CHLOROGENIC ACID,RESVERATROL,PUERARIN AND BARBALOIN IN FUNCTIONAL FOODS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

作　　者：江阳[1] 孙成均[1] 

机构地区：[1]网川大学华西公共卫生学院,成都610041

出　　处：《现代预防医学》2010年第22期4296-4298,共3页Modern Preventive Medicine

基　　金：国家“十一五”科技支撑计划课题资助(2006BAD27B02)

摘　　要：[目的]建立同时快速测定功能食品中绿原酸、白藜芦醇、葛根素和芦荟甙的高效液相色谱法。[方法]样品经甲醇超声提取,提取液经甲醇-水(20︰80)稀释并高速离心后,上清液供HPLC分析。色谱柱为Kinetex C18柱(4.6 mm×100 mm,2.6μm),柱温25℃,采用甲醇-0.15%冰乙酸水溶液作流动相,梯度洗脱,流速0.60 ml/min,紫外检测。[结果]本法线性范围上限至少为2μg,相关系数大于0.999,检出限0.15～0.72 ng,平均回收率99.6%～113%。[结论]该法简便快速、准确灵敏,可用于功能食品中绿原酸、白藜芦醇、葛根素和芦荟甙的同时测定。[Objective] To establish a method for simultaneous determination of chlorogenic acid,resveratrol,puerarin and barbaloin in functional foods by high performance liquid chromatography.[Methods] Samples were extracted with methanol ultrasonically and the extract was diluted with methanol-water（80︰20）,and then the diluent was centrifuged at 12 000 rpm.The supernatant was used for analysis.The column used was Kinetex C18 column and detected with UV detector.The mobile phase was consisted of methanol-0.15% glacial acetic acid with gradient elution,at a flow rate of 0.60 ml/ min with the col-umn temperature of 25℃.[Results] The upper limits of the liner range at least reach 2μg with the correlation coefficients above 0.999,and the detection limits were 0.15～0.72ng.The average recoveries ranged from 99.6% to 113%.[Conclusion] The method is simple,rapid,accurate and sensitive,and it is suitable for the simultaneous determination of chlorogenic acid,resveratrol,puerarin and aloin in functional foods.

关 键 词：绿原酸 白藜芦醇 葛根素 芦荟甙 高效液相色谱法 功能食品 

分 类 号：R115[医药卫生—公共卫生与预防医学]