微悬臂梁传感器对中药成分的非标记检测  

Label-Free Detection of Artemisinin and Aristolochic Acid Based on Microcantilever Sensor

作　　者：陈雁云[1] 薛长国[1] 黄渊[1] 张青川[1] 刘红[2] 王保民[3] 伍小平[1] 

机构地区：[1]中国科学技术大学中科院材料力学行为和设计重点实验室,合肥230027 [2]中国科学技术大学化学物理系,合肥230026 [3]中国农业大学农学与生物技术学院,北京100094

出　　处：《实验力学》2010年第5期529-535,共7页Journal of Experimental Mechanics

基　　金：国家重点基础研究发展计划(2006CB300404);国家自然科学基金(10627201和10732080)

摘　　要：利用微悬臂梁传感技术对两种中药成分(青蒿素与马兜铃酸)抗原抗体的特异性结合进行检测。首先用巯基化二抗将青蒿素或马兜铃酸抗体通过自组装法修饰到微悬臂梁的金面上,再加入不同浓度的青蒿素或马兜铃酸抗原,采用光杠杆原理监测微悬臂梁的实时变形。该系统能够检测出至少100ng/ml浓度的青蒿素与500ng/ml的马兜铃酸标准样品,并与ELISA实验所得的活性标准曲线趋势相对应。由于青蒿素抗体活性比马兜铃酸抗体活性强,因此检测出的青蒿素极限浓度较低。结果表明,利用微悬臂梁传感技术对中药成分的检测是可行的。A novel technology for label-free detection of Artemisinin and Aristolochic acid antigenantibody specificity binding based on microcantilever sensor platform is reported in this paper. Firstly, Sulfhydryl Artemisinin/Aristolochic acid antibody was immobilized on gold-coated side of a microcantilever by self-assembled monolayer technique. Then, the deflections of microcantilever corresponding to different levels of concentrations of Artemisinin/Aristolochic acid antigen could be real-time monitored by using optical lever technique. As a result, the sensitivity of detecting Artemisinin and Aristolochic acid is 100ng/ml and 500ng/ml, respectively, which is corresponding to the tendency of their Standard inhibition curves obtained in ELISA test. The sensitivity of detecting Artemisinin is higher due to its antibody is more active than that of Aristolochic acid antibody. Results show that it is feasible for detection of Artemisinin and Aristolochic acid by microcantilever sensor platform.

关 键 词：微悬臂梁 生物传感器 青蒿素 马兜铃酸 抗原抗体 表面应力 

分 类 号：R155.5[医药卫生—营养与食品卫生学]