铅对大鼠星型胶质细胞c-Fos和c-Jun蛋白表达影响  

Effect of lead on expressions of c-Fos and c-Jun in rat primarily cultured astrocyte cells

作　　者：张莹[1,2] 王彪[3] 赵久晗[1] 徐晓雪[1,2] 孙黎光[3] 

机构地区：[1]中国医科大学附属第一医院神经内科,辽宁沈阳110001 [2]中国医科大学神经病学研究所 [3]中国医科大学生物化学与分子生物学教研室

出　　处：《中国公共卫生》2010年第11期1392-1393,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(39970651)

摘　　要：目的探讨不同时间点低浓度铅暴露对体外原代培养的星型胶质细胞中即早基因c-Fos和c-Jun蛋白表达的影响。方法出生4～8 d Wistar大鼠脑星型胶质细胞原代培养,1.0μmol/L醋酸铅暴露不同时间后分别收集样品,应用蛋白免疫印迹法测定样品c-Fos和c-Jun蛋白表达的变化。结果 1.0μmol/L醋酸铅处理后,星型胶质细胞中c-Fos和c-Jun蛋白表达均明显降低,与对照组比较,染铅15 min时蛋白表达降低有统计学意义(P<0.05),分别比对照降低33.4%和17.3%;至染铅180 min时c-Fos和c-Jun蛋白表达降至最低,均低于对照的1/2(P<0.01)。结论低浓度染铅可使原代培养的星型胶质细胞中c-Fos和c-Jun蛋白表达持续降低,这可能与铅致大鼠学习记忆功能损害有关。Objective To observe the effect of low-level of lead on the expression of c-Fos and c-Jun in rat primarily cultured astrocyte cells.Methods The primary neural glia cells of Wistar rats of 1-4 days old were cultured.Samples of the cell culture were collected at different time after 1.0 μmol/L lead acetate exposure and the contents of c-Fos and c-Jun were assayed with Western blot method.Results The exposure of 1.0 μmol/L lead acetate could decrease the expressions of c-Fos and c-Jun remarkably compared with those of the control.There were 33.4% and 17.3% of decrease in c-Fos and c-Jun expression 15 minutes after the exposure compared to those of the control,respectively（P 0.05）.The expressions of c-Fos and c-Jun decreased to the lowest 180 minutes after the exposure and were only 1 /2 of the control（P 0.01）.Con-clusion Low-level lead exposure could result in the persistent decrease of the expressions of c-Fos and c-Jun within several hours and the decreases may relate to the damage of learning and memory capacity in rat with lead exposure.

关 键 词：铅 星型胶质细胞 即早基因 

分 类 号：R778.11[医药卫生—眼科]