聚合物驱采出水中聚丙烯酰胺的微生物联合降解作用研究  

Study on biodegradation of hydrolyzed polyacrylamide in polymer flooding produced wastewater

作　　者：常帆[1] 陈立[1] 陈亮[2] 田应天[3] 陈五岭[1] 

机构地区：[1]西北大学生命科学学院,西安710069 [2]河南工业大学生物工程学院,郑州450001 [3]中石油长庆油田公司第一采气厂,靖边718500

出　　处：《环境工程学报》2010年第11期2466-2472,共7页Chinese Journal of Environmental Engineering

基　　金：陕西省重大科技创新项目(2009ZKC04-16)

摘　　要：通过对2株细菌的培养降解实验研究聚丙烯酰胺(hydrolyzed polyacrylamide,HPAM)降解菌对水环境下聚丙烯酰胺的降解作用,讨论协同降解机理。2株降解聚丙烯酰胺的菌株假单胞菌CJ419、枯草芽孢杆菌FA16在初始30℃废水样品上培养,定期测量细菌生物量和HPAM降解率。培养30 d后CJ419和FA16对聚合物的降解率最大值分别达到30.4%和25%,而以1∶1比例的混合菌降解率最大值达到80.3%。对2株菌胞外各组分研究表明:混合菌降解HPAM的机理主要由胞外降解酶系水解聚合物侧链基团导致HPAM降解为小分子物质,同时生长过程中降解菌还会释放非蛋白还原性物质引发氧化反应共同参与HPAM降解。The effect of 2 hydrolyzed polyacrymide（HPAM）for degrading bacteria was studied in polyacrylamide.HPAM degrading bacteria CJ419 and Bacillus subtilis FA16 cultured in wastewater at 30℃.Bacterial biomass and HPAM degradation rate were investigated.After 30 d CJ419 and FA16 maximum polyacrymide degrading rates were 30.4% and 25%,respectively.Mixed bacteria reached the maximum degradation rate of 80.3%.Biochemical mechanism of degrading HPAM is that HPAM is hydrolyzed into small molecules by extracellular enzyme.Degrading bacteria also release non-protein inducing substances to participate in HPAM degradation.

关 键 词：部分水解聚丙烯酰胺 假单胞菌 枯草芽孢杆菌 复合菌 生物降解机理 胞外酶 还原性物质 

分 类 号：X172[环境科学与工程—环境科学]