一株高效微生物絮凝剂产生菌的筛选鉴定和培养条件优化  

Screening and identification of a bioflocculant-producing strain and its culture condition optimization

作　　者：黎忠[1] 张修玉[2] 

机构地区：[1]中水珠江规划勘测设计有限公司水环境与生态修复研究所,广州510610 [2]华南环境科学研究所,广州510655

出　　处：《环境工程学报》2010年第11期2515-2518,共4页Chinese Journal of Environmental Engineering

基　　金：水利部公益性行业专项科研经费(200801065)

摘　　要：从土壤中筛选到一株高效絮凝剂产生菌,经生理生化实验鉴定及16S rDNA序列分析,X14被鉴定为地衣芽胞杆菌（Bacillus licheniformis）。经培养条件优化,其最佳的培养条件为：淀粉10 g/L,氯化铵0.51 g/L,3 g/L K2HPO4和1 g/L KHPO,初始pH值为8,接种量采用1%,摇床转速采用140~160 r/min的变速控制,37℃培养48 h。A bioflocculant-producing bacterium X14 was screened,which was identified as Bacillus licheniformis by 16S rDNA sequencing and its biochemical and physiological characteristics.The optimal culture conditions for Bacillus licheniformis X14 production are starch 10 g/L,NH4Cl 0.51 g/L,3 g/L K2HPO4,1 g/L KH2PO4,initial pH 8,inoculum size 1%（V/V）,culture temperature 37℃,culture time 48 h and shaking speed adopted variable-speed control from 140 r/min to 160 r/min.

关 键 词：地衣芽孢杆菌 微生物絮凝剂 絮凝活性 培养条件优化 

分 类 号：X172[环境科学与工程—环境科学]