正畸力作用下大鼠炎性牙周组织改建及白细胞介素6的表达  

The effect of orthodontic force on the inflammatory periodontal tissue remodeling and the expression of IL-6 in rats

作　　者：钱雅婧[1] 钟良军[2] 米丛波[1] 李小兵[3] 聂晶[1] 潘旭[1] 

机构地区：[1]新疆医科大学第一附属医院口腔正畸科,乌鲁木齐830054 [2]杭州师范大学附属医院口腔科 [3]四川大学华西口腔医学院口腔正畸科

出　　处：《中华口腔医学研究杂志（电子版）》2010年第5期15-19,共5页Chinese Journal of Stomatological Research(Electronic Edition)

基　　金：新疆医科大学青年启动基金(2009-QN-16)

摘　　要：目的探讨正畸力及炎症条件下白细胞介素6(IL-6)在大鼠牙周组织内的表达分布及牙槽骨高度的改变,研究正畸力和炎症对大鼠牙周组织改建的联合作用。方法将60只SD大鼠随机分为空白对照组(P1组)、加力对照组(P2组)、炎症对照组(P3组)和炎症加力组(P4组)。施加50g近中向正畸力于P2组大鼠的左侧上颌第一磨牙,同时建立实验性牙周炎于P3、P4组大鼠。实验性牙周炎动物模型建模2周后,施加50g近中向正畸力于P4组大鼠左侧上颌第一磨牙。运用免疫组织化学及组织形态分析法观测各组大鼠上颌第一磨牙IL-6表达量及牙槽骨吸收情况。结果正常大鼠(P1组)牙周组织中IL-6呈低浓度表达;P2组大鼠牙周组织中IL-6的表达与正畸加力时间相关,受力后第3天达到高峰,之后开始下降,10d后基本恢复正常;在炎症条件下,P3组大鼠牙周组织中IL-6的表达增加;在正畸力和炎症的联合作用下,P4组与P2组相比较,IL-6的表达较强,在受力后第5天达到峰值后开始缓慢下降。与P1组比较,P2组近中牙槽骨中未见明显丧失;而与P1、P2组相比较,近中牙槽骨在P3和P4组中可见明显丧失。结论正畸力和炎症刺激物均可引发IL-6的表达。在炎症条件下,正畸力可促使IL-6的表达进一步增加。但是,只要彻底清除炎性刺激物,正畸力不会导致牙槽骨的明显丧失。Objective To study the combined effect of orthodontic force and inflammation on the periodontal tissue remodeling by evaluating the expression and distribution of IL-6 in periodontal tissue and the change of the alveolar bone of rats under the condition of orthodontic force and inflammation. Methods 60 SD rats were randomly divided into controlled group（P1）,orthodontic tooth movement group （P2）,periodontitis group （P3） and orthodontic tooth movement with periodontitis group （P4）. 50 gram orthodontic force was loaded on the maxillary left first molars of 25 rats in P2. Experimental periodontitis were established in the other 30 rats of P3,P4. 2 weeks after the establishment of the experimental periodontitis,50 gram orthodontic force was loaded on the maxillary left first molars of P4. Immunohistochemistry and histomorphometric analysis was performed to measure the expression of IL-6 and the loss of alveolar bone of the 4 groups. Results The IL-6 expressed in a low concentration in P1. The expression of IL-6 in P2 was related with the time of orthodontic treatment,which was observed to reach a maximum on day 3 and decline thereafter to the normal standard on day 10. The expression level of IL-6 in P3 increased when periodontitis occurred. Compared with P2,the expression level of IL-6 in P4 increased and reached the maximum on day 5 and then declined slowly under the combined effect of orthodontic force and inflammation. Compared with P1,no obvious alveolar bone loss was detected in the mesial side of P2; Compared with P1 and P2,obvious alveolar bone loss was detected in the mesial side of P3 and P4. Conclusions Both orthodontic force and inflammatory stimulus can stimulate the expression of IL-6. When accompanied by periodontitis,orthodontic force can evoke an uplifting expression of IL-6. But orthodontic force can not cause severe alveolar bone loss as long as the local inflammatory stimulus was eliminated.

关 键 词：正畸牙移动 牙周炎 白细胞介素6 牙槽骨改建 SD大鼠 

分 类 号：R783.5[医药卫生—口腔医学]