Developmental expression amphioxus Branchiostoma pattern of calmodulin gene in belcheri tsingtauense  

作　　者：LUAN Jing GENG Jinpei FANG Shaoqing SU Zhiping ZHANGJingxuan XU Hongyan WANGYing LUMin YIN Weili MENG Guangxiao 

机构地区：[1]Department of Marine Biology, Ocean university of China, Qingdao 266003, China [2]Yantai Entry-Exit Inspection and Quarantine Bureau, Yantai 264000, China

出　　处：《Chinese Journal of Oceanology and Limnology》2010年第6期1221-1226,共6页中国海洋湖沼学报（英文版）

基　　金：Supported in part by Shandong Entry-Exit Inspection and Quarantine Bureau （SK200807） We thank Dr. LI Xiangning, who works in the CSR （Center for Scientific Review） of NIH （National Institutes of Health）, USA, for his critical reading of the manuscript.

摘　　要：We investigated the developmental expression pattern of AmphiCaM in cephalochordate amphioxus （Branchiostoma belcheri tsingtauense）. We cultured and sampled the animals at different developmental stages （eggs and larvae）, and used in-situ hybridization and northern blotting to document the spatial and temporal changes in AmphiCaM expression. The alimentary tract dominates the development from the late neurula stage to the adult stage. AmphiCaM expression increased significantly in the alimentary tract during the late neurula stage and remained elevated in the adults. Our results indicate that AmphiCaM is involved in the differentiation of the alimentary tract in amphioxus; and furthermore, provide an insight into the change in function of CaM genes during evolution.我们在头索动物文昌鱼(Branchiostoma belcheri tsingtauense ) 调查了 AmphiCaM 的发展表示模式。我们有教养并且在不同发展阶段(鸡蛋和幼虫) 取样了动物，并且在原处使用了杂交并且在 AmphiCaM 记录空间、时间的变化的北弄污表示。滋养的道从迟了的神经轴胚阶段统治发展到成年阶段。AmphiCaM 表示在迟了的神经轴胚阶段期间在滋养的道显著地增加了并且仍然保持在成年人提高了。我们的结果显示 AmphiCaM 在文昌鱼涉及滋养的道的区别；并且而且，在进化期间在凸轮基因的功能提供卓见进变化。

关 键 词：AMPHIOXUS CALMODULIN expression evolution 

分 类 号：Q959.287[生物学—动物学] S828.5