利用气相色谱法同时测定番茄叶片中的信号物质水杨酸和茉莉酸  

Simultaneous Quantification of Jasmonic and Salicylic Acids in Tomato Plants by Gas Chromatography

作　　者：宋圆圆[1] 徐建峰[1] 梁笑婷[1] 苏贻娟[1] 谢丽君[1] 曾任森[1] 

机构地区：[1]华南农业大学/农业部生态农业重点开放实验室/热带亚热带生态研究所,广东广州510642

出　　处：《江西农业大学学报》2010年第5期1056-1060,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(30870390)

摘　　要：茉莉酸(JA)和水杨酸(SA)是植物中重要的内源激素和防御反应信号分子,快速而准确地定量测定植物内源SA和JA含量非常重要。以番茄叶片作为材料,研究利用气相色谱(GC)同时测定植物内源JA和SA的含量。利用丙酮/柠檬酸(50mmol/L)(体积分数=7/3)和乙酸乙酯提取番茄叶片中的JA和SA,提取液经N2吹干后用三甲基硅烷化重氮甲烷甲酯化,然后用顶空-固相微萃取(HS-SPME)方法进行收集,正己烷洗脱后进行气相色谱分析,用氢离子火焰检测器(FID)进行检测,梯度升温程序为60℃(1min)至250℃,15℃/min,250℃,3min。从样品中得到与茉莉酸甲酯(MeJA)和水杨酸甲酯(MeSA)标准品完全吻合的色谱峰。该方法可以广泛用于同时测定植物内源的SA和JA含量。Jasmonic and salicylic acids are important phytohormones and plant defensive signaling compounds.Here a new method was developed for the simultaneous quantification of the two signaling compounds in tomato leaves by using headspace-solid phase microextraction（HS-SPME） and gas chromatography with hydrogen ion flame detector（FID）.Leaf samples were extracted by mixture of acetone and citric acid（50 mmol/L）（V/V=7/3）,and ethyl acetate.Then the supernatant was dried by N2 and subsequently methylated with trimethylsilyldiazomethane.The volatilized compounds were collected by using headspace-solid phase microextraction on Tenax adsorbents,and eluted with n-hexane.Eluted samples were analyzed by using GC with hydrogen ion flame detector（FID）.The temperature gradient was increased from 60 ℃（1 min） to 250 ℃ in a rate of 15 ℃/min and held on 3 min at 250 ℃.The final chromatographic peaks of JA and SA in the samples were identical to the authentic compounds.This method will be useful for simultaneous quantification of JA and SA in plants.

关 键 词：气相色谱 信号分子 茉莉酸 水杨酸 甲酯化 顶空-固相微萃取 

分 类 号：Q948[生物学—植物学]