高羊茅EST-SSR标记的开发及品种鉴定  

Development of EST-SSR Markers for Cultivar Identification of Festuca arundinacea

作　　者：段乃彬[1] 王建成[1] 于天祥[2] 孔素萍[3] 李群[1] 张文兰[1] 戴双[1] 

机构地区：[1]山东省农业科学院作物研究所,山东济南250100 [2]嘉兴检验检疫局,浙江嘉兴314001 [3]山东省农业科学院蔬菜研究所,山东济南250100

出　　处：《山东农业科学》2010年第10期7-10,共4页Shandong Agricultural Sciences

基　　金：嘉兴市科技计划项目"嘉兴市进口草坪草种--高羊茅品种鉴定"(2007AY2004)资助

摘　　要：利用EST-SSR分子标记对11份高羊茅（Festuca arundinacea）材料进行了品种鉴定研究。在优化DNA提取方法、扩增体系的基础上对50对EST-SSR引物进行筛选,结果表明：有42对引物可以在高羊茅上得到有效扩增,对11份高羊茅品种的鉴定结果表明,其中12对引物具有良好的多态性,占28.6%;共检测到86个等位基因,平均每个位点的等位基因数为4.5个,变幅为3-12。从谱带类型上看：S3和S44引物扩增的谱带类型最少（2种）,多态性百分率最低,为18.2%;S11引物扩增的谱带类型最多（9种）,多态性百分率最高,为81.8%;共扩增到多态性谱带65种,平均多态百分率为59.1%。In this study,EST-SSR markers were developed for identification of 11 Festuca arundinacea cultivars at the molecular level.Fifty pairs of EST-SSR primers were screened based on the optimization of DNA extracting method and amplification system.The results showed that 42 pairs of EST-SSR primers had effective amplification,and twelve pairs of them had better polymorphism,which occupied 28.6%.A total of 86 alleles were detected with the mean of 4.5 alleles ranging from 3 to 12.Sixty-five types of polymorphic bands were amplified,and the ratio of polymorphism was 59.1%.The types of polymorphic bands amplified by the primers S3 and S44 were the least with the ratio of polymorphism as 18.2%,while those of S11 were the most with the ratio of polymorphism as 81.8%.

关 键 词：高羊茅 品种鉴定 EST-SSR 

分 类 号：Q789[生物学—分子生物学] S688.403.7[农业科学—观赏园艺]