长白山牛皮杜鹃组培苗与野生苗药用成分含量分析  

Analysis of Medical Components in the Tissue Culture Plantlets and Wild Seedling of Rhododendron chrysanthum Pall from Changbai Mountain

作　　者：于秋艳[1] 宗成文[1] 赵金伟[1] 韩苗苗[1] 曹后男[1] 

机构地区：[1]延边大学农学院,吉林龙井133400

出　　处：《湖北农业科学》2010年第11期2892-2894,共3页Hubei Agricultural Sciences

基　　金：延边大学科技发展计划项目[延大科合字(2009)第13号];延边大学农学院研究生创新课题

摘　　要：采用HPLC法与紫外分光光度法分别测定了长白山牛皮杜鹃组培苗与野生苗中主要药用成分芦丁与单宁酸的含量。结果表明,芦丁在野生苗两年生老叶中含量最高,为2.54mg/g,野生苗嫩叶含量为1.68mg/g,愈伤组织含量为0.16mg/g,组培苗含量为0.08mg/g,组培叶含量为0.03mg/g,根中未检测到芦丁成分。单宁酸在野生苗老叶中含量最高,为91.54mg/g,野生苗嫩叶含量为45.87mg/g,野生苗根中为24.58mg/g,愈伤组织中含量为13.51mg/g,组培苗中含量为10.32mg/g,组培叶中含量为17.46mg/g。野生苗中芦丁与单宁酸含量均高于组培苗。The contents of rutin and tannins in the tissue culture plantlets and wild Seedling of Rhododendron chrysanthum Pall from Changbai Mountain were measured by HPLC and ultraviolet spectrophotometry.The results revealed that the contents of rutin in the old and tender leaves of 2-years wild one were 2.54 mg/g and 1.68 mg/g respectively;the rutin in callus,tissue culture plantlet and its tender leaves were 0.16,0.08 and 0.03 mg/g respectively;no rutin in root was detected.The contents of tannins in wild old and tender leaves were 91.54 mg/g and 45.87 mg/g respectively.24.58 mg/g tannins in root and 13.51 mg/g in callus were detected.Tannins in tissue culture plantlet and leaves were 10.32 mg/g and 17.46 mg/g respectively.In conclution,the level of rutin and tannins in wild plantlets was higher than that of tissue culture plantlets.

关 键 词：牛皮杜鹃 组培苗 野生苗 药用成分 

分 类 号：O657.72[理学—分析化学] O657.3[理学—化学]